RT Journal Article
T1 Characterization of the Microbial Population Inhabiting a Solar Saltern Pond of the Odiel Marshlands (SW Spain)
A1 Gómez Villegas, Patricia
A1 Vigara Fernández, Javier
A1 León Bañares, Rosa María
AB The solar salterns located in the Odiel marshlands, in southwest Spain, are an excellentexample of a hypersaline environment inhabited by microbial populations specialized in thrivingunder conditions of high salinity, which remains poorly explored. Traditional culture-dependenttaxonomic studies have usually under-estimated the biodiversity in saline environments due to thedifficulties that many of these species have to grow at laboratory conditions. Here we compare twomolecular methods to profile the microbial population present in the Odiel saltern hypersaline waterponds (33% salinity). On the one hand, the construction and characterization of two clone PCRamplified-16S rRNA libraries, and on the other, a high throughput 16S rRNA sequencing approachbased on the Illumina MiSeq platform. The results reveal that both methods are comparable forthe estimation of major genera, although massive sequencing provides more information about theless abundant ones. The obtained data indicate that Salinibacter ruber is the most abundant genus,followed by the archaea genera, Halorubrum and Haloquadratum. However, more than 100 additionalspecies can be detected by Next Generation Sequencing (NGS). In addition, a preliminary study totest the biotechnological applications of this microbial population, based on its ability to produce andexcrete haloenzymes, is shown.
PB MDPI
SN 1660-3397
YR 2018
FD 2018-09
LK http://hdl.handle.net/10272/18232
UL http://hdl.handle.net/10272/18232
LA eng
NO Gómez Villegas, P., Vigara Fernández, J., & León Bañares, R. (2018). Characterization of the Microbial Population Inhabiting a Solar Saltern Pond of the Odiel Marshlands (SW Spain). Marine Drugs, 16(9), 332. DOI: https://doi.org/10.3390/md16090332
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026