RT Journal Article
T1 Impact of heavy metals in the microalga Chlorella sorokiniana and assessment of its potential use in cadmium bioremediation
A1 León Vaz, Antonio
A1 León Bañares, Rosa María
A1 Giráldez Díaz, Inmaculada
A1 Vega Piqueres, José María
A1 Vigara Fernández, Javier
AB The chlorophyte microalga Chlorella sorokiniana was tested for the bioremediation of heavy metals pollution. It was cultured with different concentrations of Cu2+, Cd2+, As (III) and As (V), showing a significant inhibition on its growth at concentrations of 500 µM Cu2+, 250 µM Cd2+, 750 µM AsO33- and 5 mM AsO43- or higher. Moreover, the consumption of ammonium was also studied, showing significant differences for concentrations higher than 1 mM of Cu2+ and As (III), and 5 mM of As (V). The determination of intracellular heavy metals concentration revealed that Chlorella sorokiniana is an outstanding Cd accumulator organism, able to accumulate 11,232 mg kg−1 of Cd, and removing 65% of initial concentration of this heavy metal. Finally, antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), and enzymes involved in the production of glutamate and cysteine, such as glutamine syntethase (GS), glutamate dehydrogenase (GDH), O-acetylserine (thiol) lyase (OASTL) and NAD-isocitrate dehydrogenase (NAD-IDH) were studied both at gene expression and enzymatic activity levels. These enzymes exhibited different grades of upregulation, especially in response to Cd and As stress. However, GS expression was downregulated when Chlorella sorokiniana was cultured in the presence of these heavy metals.
PB Elsevier
SN 0166-445X
SN 1879-1514 (electrónico)
YR 2021
FD 2021-10
LK http://hdl.handle.net/10272/21179
UL http://hdl.handle.net/10272/21179
LA eng
NO León-Vaz, A., León, R., Giráldez, I., Vega, J. M., & Vigara, J. (2021). Impact of heavy metals in the microalga Chlorella sorokiniana and assessment of its potential use in cadmium bioremediation. In Aquatic Toxicology (Vol. 239, p. 105941). Elsevier BV. https://doi.org/10.1016/j.aquatox.2021.105941
NO This work was supported in part by research grants from the Euro-pean governments (INTERREG VAPOCTEP-2014-2020; 0055_ALGAR-ED_PLUS_5_E), the Operative FEDER Program-Andalucía 2014-2020,the University of Huelva (UHU-1257518), and by the European Regional Development Fund through the Agencia Estatal de Investigaci ́on grant(PID 2019-110438RB-C22)
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026