RT Journal Article
T1 Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
A1 Fuentes Cordero, Juan Luis
A1 Huss, Volker A. R.
A1 Montero Lobato, Zaida
A1 Torronteras Santiago, Rafael
A1 Cuaresma Franco, María
A1 Garbayo Nores, Inés
A1 Vílchez Lobato, Carlos
AB The genus Coccomyxa comprises green microalgae, which can be found worldwide in remarkably versatile aquatic and terrestrial ecosystems including symbiotic associations with a number of different hosts. In this study, we describe a new species, Coccomyxa onubensis, based on 18S and ITS ribosomal DNA (rDNA) sequence data. Coccomyxa onubensis was isolated from acidic water, and its ability to adapt to a wide range of acidic and alkaline pH values and to high salinity wasanalyzed. The long-termadaptation capacity of the microalga to such extreme conditions was evaluated by performing continuous repeated batches at selected salt concentrations and pHvalues. Adapted cultures of C. onubensis were found to yield high biomass productivities from pH 2.5 to 9, with maximum yields at acidic pH between 2.5 and 4.5. Moreover, C. onubensis was also found to adapt to salinities as high as 0.5 M NaCl, reaching biomass productivities that were similarto those of control cultures. Ultrastructural analysis by transmission electron microscopy of C. onubensis cells adapted to high salinity showed a robust response to hyperosmotic shock.Thus, C. onubensis was found to be acidotolerant and halotolerant. High biomass productivity over a wide range of pH and salinities denotes C. onubensis as an interesting candidate for various biotechnological applications including outdoor biomass production.
SN 1573-5176 (electrónico)
YR 2016
FD 2016-06-21
LK https://hdl.handle.net/10272/24958
UL https://hdl.handle.net/10272/24958
LA eng
NO Fuentes, J.L., Huss, V.A.R., Montero, Z., Torronteras, R., Cuaresma, M., Garbayo, I., Vílchez, C. (2016). Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae). Journal Applied Phycology 28(6): 3269-3279
NO The authors want to acknowledge the support from Junta de Andalucía (Grant no. AGR-4337) and CEIMAR (PhD Grant forJuan Luis Fuentes) and the technical support from Enrique Chaguaceda, María J. Vílchez, Gloria Blanco (Central Services, CIDERTA), and BioAvan SL. This is contribution No. 131 from the CEIMAR Journal Series.
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026