RT Journal Article
T1 Effects of Dietary Phenylalanine and Tyrosine Supplements on the Chronic Stress Response in the Seabream (Sparus aurata)
A1 Salamanca de las Nieves, Natalia
A1 Moreno, Oscar
A1 Giráldez Díaz, Inmaculada
A1 Rosa Lucas, Ignacio de la
A1 Herrera Rodríguez, Marcelino
AB The increase of aquaculture production is associated with a growing interest in improving physiological status and welfare in fish. For this reason, the search for strategies for mitigating stress has been intensified, with one of these strategies being food supplementation with different amino acids (AA). The objective of this study was to evaluate the effects of dietary phenylalanine (Phe) and tyrosine (Tyr) supplements on the endocrine and physiological state of seabreams (Sparus aurata) subjected to chronic stress. The fish were stocked at 30 fish/tank in a recirculation aquatic system, fed one control diet and two diets supplemented with 5% Phe or Tyr for 90 days. Blood was drawn from 10 fish per tank every 30 days, and the weight and length were measured every 15 days. At the end of the experiment, length/weight of the fish were measured, and they were sacrificed for the extraction of blood, head kidney, liver, and brain. Classic plasma stress markers (glucose, lactate, proteins, and cortisol), as well as hormones derived from Phe and Tyr (adrenaline, norepinephrine, and dopamine) and the accumulation of AA were analyzed. Fish fed with diets supplemented with Phe or Tyr showed a reduction in various stress markers and physiological parameters. In addition, the stress condition favored a mobilization of AA toward the tissues, especially in supplemented diets, so this excess of AA could be used as an energy substrate to cope with stress.
PB Frontiers Media
SN 1664-042X (electrónico)
YR 2022
FD 2022
LK https://hdl.handle.net/10272/23344
UL https://hdl.handle.net/10272/23344
LA eng
NO Salamanca, N., Moreno, O., Giráldez, I., Morales, E., de la Rosa, I., & Herrera, M. (2022). Effects of Dietary Phenylalanine and Tyrosine Supplements on the Chronic Stress Response in the Seabream (Sparus aurata). In Frontiers in Physiology (Vol. 12). Frontiers Media SA. https://doi.org/10.3389/fphys.2021.775771
NO This research was funded by the projects INIA RTA2015-00025-C03-01 and 0750_AQUA_AMBI_2_5_P (INTERREG VA). MH and NS belong to the Fish Welfare and Stress Network (AGL2016-81808-REDT), supported by the Agencia Estatal de Investigación (AEI, MICINN, Spanish Government). NS’s predoc contract is confinanced by the European Social Fund (FSE) through the call “Ayudas para contratos predoctorales para la formación de doctores 2017” from the AEI.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026