RT Journal Article
T1 Identifying Chloris Species from Cuban Citrus Orchards and Determining Their Glyphosate-Resistance Status
A1 Bracamonte, Enzo R.
A1 Fernández Moreno, Pablo T.
A1 Bastida Milián, Fernando
A1 Osuna, María D.
A1 Alcántara de la Cruz, Ricardo
A1 Cruz Hipólito, Hugo E.
A1 De Prado, Rafael
AB The Chloris genus is a C4 photosynthetic species mainly distributed in tropical andsubtropical regions. Populations of three Chloris species occurring in citrus orchards fromcentral Cuba, under long history glyphosate-based weed management, were studiedfor glyphosate-resistant status by characterizing their herbicide resistance/tolerancemechanisms. Morphological and molecular analyses allowed these species to beidentified as C. ciliata Sw., Chloris elata Desv., and Chloris barbata Sw. Based on theglyphosate rate that causes 50%mortality of the treated plants, glyphosate resistance (R)was confirmed only in C. elata, The R population was 6.1-fold more resistant comparedto the susceptible (S) population. In addition, R plants of C. elata accumulated 4.6-foldless shikimate after glyphosate application than S plants. Meanwhile, populations ofC. barbata and C. ciliata with or without glyphosate application histories showed similarLD50 values and shikimic acid accumulation rates, demonstrating that resistance toglyphosate have not evolved in these species. Plants of R and S populations of C. elatadiffered in 14C-glyphosate absorption and translocation. The R population exhibited27.3-fold greater 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) activity thanthe S population due to a target site mutation corresponding to a Pro-106-Sersubstitution found in the EPSPS gene. These reports show the innate tolerance toglyphosate of C. barbata and C. ciliata, and confirm the resistance of C. elata to thisherbicide, showing that both non-target site and target-site mechanisms are involved inits resistance to glyphosate. This is the first case of herbicide resistance in Cuba.
PB University of Bern, Switzerland
SN 1664-462X
YR 2017
FD 2017-11
LK http://hdl.handle.net/10272/16086
UL http://hdl.handle.net/10272/16086
LA eng
NO Enzo R. Bracamonte, Pablo T. Fernández Moreno, Fernando Bastida, María D. Osuna, Ricardo Alcántara de la Cruz, Hugo E. Cruz Hipólito, Rafael De Prado. Identifying Chloris Species from Cuban Citrus Orchards and Determining Their Glyphosate-Resistance Status. Frontiers in Plant Science, November 2017, Volume 8, Article 1977. ESSN: 1664-462X. DOI 10.3389/fpls.2017.01977
NO This work was funded by the AGL2016-78944-R (MICINN, Spain), GR15112 (Research Group AGA001 - Junta de Extremadura, Spain) and 242088 (CONACYT, Mexico) projects.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026