RT Journal Article
T1 Nitrate modulates the physiological tolerance responses of the halophytic species Sarcocornia fruticosa to copper excess
A1 Valle Romero, Pedro
A1 Castellanos Verdugo, Eloy Manuel
A1 Luque Palomo, Carlos Javier
A1 Flores Duarte, Noris J.
A1 Romano Rodríguez, Elena
A1 Redondo Gómez, Susana
A1 Rodríguez Llorente, Ignacio D.
A1 Pajuelo Domínguez, Eloisa
A1 Mateos Naranjo, Enrique
AB Coexistence impact of pollutants of different nature on halophytes tolerance to metal excess has not been thoroughly examined, and plant functional responses described so far do not follow a clear pattern. Using the Cu-tolerant halophyte Sarcocornia fruticosa as a model species, we conducted a greenhouse experiment to evaluate the impact of two concentration of copper (0 and 12 mM CuSO4) in combination with three nitrate levels (2, 14 and 50 mM KNO3) on plant growth, photosynthetic apparatus performance and ROS-scavenging enzymes system. The results revealed that S. fruticosa was able to grow adequately even when exposed to high concentrations of copper and nitrate. This response was linked to the plant capacity to uptake and retain a large amount of copper in its roots (up to 1500 mg kg−1 Cu), preventing its transport to aerial parts. This control of translocation was further magnified with nitrate concentration increment. Likewise, although Cu excess impaired S. fruticosa carbon assimilation capacity, the plant was able to downregulate its light-harvesting complexes function, as indicated its lowers ETR values, especially at 12 mM Cu + 50 mM NO3. This downregulation would contribute to avoid excess energy absorption and transformation. In addition, this strategy of avoiding excess energy was accompanied by the upregulation of all ROS-scavenging enzymes, a response that was further enhanced by the increase in nitrate concentration. Therefore, we conclude that the coexistence of nitrate would favor S. fruticosa tolerance to copper excess, and this effect is mediated by the combined activation of several tolerance mechanisms.
PB Elsevier
SN 0981-9428
SN 1873-2690 (electrónico)
YR 2024
FD 2024-03
LK https://hdl.handle.net/10272/23820
UL https://hdl.handle.net/10272/23820
LA eng
NO Valle-Romero, P., Castellanos, E. M., Luque, C. J., Flores-Duarte, N. J., Romano-Rodríguez, E., Redondo-Gómez, S., Rodríguez-Llorente, I. D., Pajuelo, E., & Mateos-Naranjo, E. (2024). Nitrate modulates the physiological tolerance responses of the halophytic species Sarcocornia fruticosa to copper excess. In Plant Physiology and Biochemistry (Vol. 210, p. 108569). Elsevier BV. https://doi.org/10.1016/j.plaphy.2024.108569
NO This work has been financed by the Campus of International Excellenceof the Sea (CEIMAR) within the framework of the “Plan Propio2021”, by the company “Halófitas Onuba S.L” and supported by thegrant project PID2021-124750NB-I00 financiado por MICIU/AEI/10.13039/501100011033 y por FEDER, UE, and TED2021-131605B–I00, MICIU/AEI/10.13039/501100011033 y por la UniónEuropea NextGenerationEU/ PRTR. We also like to thank the Universityof Seville Greenhouse Research General Service (SGI, CITIUS) forproviding facilities and equipment. We also appreciate the valuablefeedback from reviewers insofar as it has helped us improve and enrichour work.
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jul 2026