RT Journal Article
T1 Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin
A1 Madrid, Cristina
A1 Nieto Liñán, José Miguel
A1 Paytubi, Sònia
A1 Falconi, Maurizio
A1 Gualerzi, Claudio O.
A1 Juárez, Antonio
AB Proteins H-NS and Hha form a nucleoprotein complex that modulates expression of the thermoregulated hlyoperon of Escherichia coli. We have been able to identify two H-NS binding sites in the hly regulatory region.One of them partially overlaps the promoter region (site II), and the other is located about 2 kbp upstream (siteI). In contrast, Hha protein did not show any preference for specific sequences. In vitro, temperature influencesthe affinity of H-NS for a DNA fragment containing both binding sites and H-NS-mediated repression of hlyoperon transcription. Deletion analysis of the hly regulatory region confirms the relevance of site I forthermoregulation of this operon. We present a model to explain the temperature-modulated repression of thehly operon, based on the experiments reported here and other, preexisting data.
PB American Society for Microbiology
SN 0021-9193
SN 1098-5530 (electrónico)
YR 2002
FD 2002-09
LK http://hdl.handle.net/10272/7491
UL http://hdl.handle.net/10272/7491
LA eng
NO Madrid, C., Nieto Liñán, J.M.,  Paytubi, S., Falconi, M., Gualerzi, C.O.: "Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin". Journal of Bacteriology. Vol. 184, No. 18, págs. 5058–5066 (2002). ISSN  0021-9193
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026