RT Journal Article
T1 Variability of Omega-3/6 Fatty Acid Obtained  Through Extraction-Transesterification Processes  from Phaeodactylum tricornutum
A1 Ruiz Domínguez, María del Carmen
A1 Toledo, Constanza
A1 Órdenes, Daniel
A1 Vílchez Lobato, Carlos
A1 Ardiles, Paula
A1 Palma, Jenifer
A1 Cerezal, Pedro
A1 Ruiz Domínguez, María del Carmen
AB The effect of direct transesterification methods on the omega-3/6 composition of extracts from Phaeodactylum tricornutum was studied. The aim of this work was to identify an extraction method which allowed to obtain the most suitable profile of fatty acids in terms of its potential benefits to health, particularly if further used in the food industry. Seven methods using acids, alkalis, and heterogeneous-catalysts, (namely methods from 1 to 7, abbreviated as M1-M7) were performed to determine α-linolenic (ALA), linoleic (LA), docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids. The composition of fatty acids was in all cases characterized by the major abundance of palmitic (23.95–34.08%), palmitoleic (30.94–35.56%), oleic acids (3.00–7.41%), and EPA (0.5–6.45%). Unsaturated fatty acids extraction yield was higher with a two-step transesterification process (M6, 63.65%). The total fatty acid methyl ester content (FAME) obtained with acid-transesterification (M1) reached about 21% wt, and 60% w/w total lipids. ALA higher relative content (ALA/LA ratio) was obtained when a lipid pre-extraction step was performed prior to acid-catalysis (M4). The transesterification method based on alkali-catalyst (M3, KOH catalyst) led to obtain higher DHA relative contents (DHA/EPA ratio up to 0.11), although its FAME content was 3.75-fold lower than that obtained with acid-transesterification (M1). Overall, this study shows that direct transesterification with alkali-catalyst (M3) improves the determination of PUFA content from the diatom through a more efficient transesterification-based extraction process, and thus allow to assess the value of the biomass more accurately for application in the food industry.
PB Slovenian Chemical Society
SN 1580-3155 (electrónico)
SN 1318-0207
YR 2021
FD 2021
LK http://hdl.handle.net/10272/20360
UL http://hdl.handle.net/10272/20360
LA eng
NO Ruiz-Domínguez, M. C., Toledo, C., Órdenes, D., Vílchez, C., Ardiles, P., Palma, J., & Cerezal, P. (2021). Variability of Omega-3/6 Fatty Acid Obtained Through Extraction-Transesterification Processes from Phaeodactylum tricornutum. In Acta Chimica Slovenica (Vol. 68, Issue 3, pp. 629–637). Slovenian Chemical Society. https://doi.org/10.17344/acsi.2020.6621
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026