RT Journal Article
T1 Haloferax mediterranei Cells as C50 Carotenoid Factories
A1 Giani, Micaela
A1 Montero Lobato, Zaida
A1 Garbayo Nores, Inés
A1 Vílchez Lobato, Carlos
A1 Vega Piqueres, José María
A1 Martínez Espinosa, María Rosa
AB Haloarchaea produce C50 carotenoids such as bacterioruberin, which are of biotechnologicalin-terest. This study aimed to analyze the effect of different environmental and nutritional conditionson the cellular growth and dynamics of carotenoids accumulation in Haloferax mediterranei. Themaximum production of carotenoids (40 μg·mL−1) was obtained during the stationary phase ofgrowth, probably due to nutrient-limiting conditions (one-step culture). By seven days of culture,1 mL culture produced 22.4 mg of dry weight biomass containing 0.18 % (w/w) of carotenoids. Onthe other hand, carbon-deficient cultures (low C/N ratio) were observed to be optimum for C50bacterioruberin production by Hfx. mediterranei, but negatively affected the growth of cells. Thus, atwo-steps process was evaluated for optimum carotenoids yield. In the first step, a nutri-ent-repletedculture medium enabled the haloarchaea to produce biomass, while in the second step, the biomasswas incubated under osmotic stress and in a carbon-deficient medium. Under the conditions used,the obtained biomass contained 0.27% (w/w) of carotenoids after seven days, which accounts for58.49 μg·mL−1 of carotenoids for a culture with turbidity 14.0.
PB MDPI
SN 1660-3397
YR 2021
FD 2021-02
LK http://hdl.handle.net/10272/19611
UL http://hdl.handle.net/10272/19611
LA eng
NO Giani, M., Montero Lobato, Z., Garbayo Nores, I., Vílchez Lobato, C., Vega v, J. M., & Martínez Espinosa, R. M. (2021). Haloferax mediterranei Cells as C50 Carotenoid Factories. Marine Drugs, 19(2), 100. DOI: https://doi.org/10.3390/md19020100
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026