RT Journal Article
T1 Targeted and untargeted metabolomic analysis of Procambarus clarkii exposed to a “chemical cocktail” of heavy metals and diclofenac
A1 Rodríguez Moro, Gema
A1 Román Hidalgo, Cristina
A1 Ramírez Acosta, Sara
A1 Aranda Merino, Noemí
A1 Gómez Ariza, José Luis
A1 Abril, Nieves
A1 Bello López, Miguel Ángel
A1 Fernández Torres, Rut
A1 García Barrera, Tamara
AB Water pollution poses an important problem, but limited information is available about the joined effects of xenobiotics of different chemical groups to evaluate the real biological response. Procambarus clarkii (P. clarkii) has been demonstrated to be a good bioindicator for assessing the quality of aquatic ecosystems. In this work, we studied the bioaccumulation of cadmium (Cd), arsenic (As) and diclofenac (DCF) in different tissues of P. clarkii during 21 days after the exposure to a “chemical cocktail” of As, Cd and DCF, and until 28 days considering a depuration period. In addition, a combined untargeted and targeted metabolomic analysis was carried out to delve the metabolic impairments caused as well as the metabolization of DCF. Our results indicate that As and Cd were mainly accumulated in the hepatopancreas followed by gills and finally abdominal muscle. As and Cd show a general trend to increase the concentration throughout the exposure experience, while a decrease in the concentration of these elements is observed after 7 days of the depuration process. This is also the case in the abdominal muscle for Cd, but not for As and DCF, which increased the concentration in this tissue in the depuration phase. The hepatopancreas showed the greatest number of metabolic pathways affected. Thus, we observed a crucial bioaccumulation of xenobiotics and impairments of metabolites in different tissues. This is the first study combining the exposure to metals and pharmaceutically active compounds in P. clarkii by untargeted metabolomics including the biotransformation of DCF.
PB Elsevier
SN 0045-6535
YR 2021
FD 2021
LK http://hdl.handle.net/10272/20446
UL http://hdl.handle.net/10272/20446
LA eng
NO G. Rodríguez-Moro, C. Román-Hidalgo, S. Ramírez-Acosta, N. Aranda-Merino, J.L. Gómez-Ariza, N. Abril, M.A. Bello-López, R. Fernández-Torres, T. García-Barrera,Targeted and untargeted metabolomic analysis of Procambarus clarkii exposed to a “chemical cocktail” of heavy metals and diclofenac,Chemosphere, Volume 293, 2022, 133410,ISSN 0045-6535, https://doi.org/10.1016/j.chemosphere.2021.133410
NO La contaminación del agua plantea un problema importante, pero se dispone de información limitada sobre los efectos conjuntos de xenobióticos de diferentes grupos químicos para evaluar la respuesta biológica real. Procambarus clarkii (P. clarkii) ha demostrado ser un buen bioindicador para evaluar la calidad de los ecosistemas acuáticos. En este trabajo, se ha estudiado la bioacumulación de cadmio (Cd), arsénico (As) y diclofenaco (DCF) en diferentes tejidos durante 21 días después de la exposición a un “cóctel químico” de As, Cd y DCF, y hasta 28 días considerando un período de purificación. Además, se llevó a cabo un análisis metabolómico dirigido y no dirigido  para profundizar en las alteraciones metabólicas provocadas así como en la metabolización del DCF.
NO This work was supported by the coordinated projects PGC 2018- 096608-B-C21 and PGC 2018-096608-B-C22 from the Spanish Ministry of Science and innovation (MCIN). (Generacion ´ del Conocimiento. MCIN/AEI/10.13039/501100011033/FEDER “Una manera de hacer Europa”). Authors are grateful to FEDER (European Community) for financial support, Grant UNHU13-1E-1611. Rodríguez-Moro, G. thanks to Plan Andaluz de Investigacion, ´ Desarrollo e Innovacion ´ (PAIDI 2020) and Fondo Social Europeo for a postdoctoral grant (DOC_01115). Au thors are grateful to Servicio General de Investigacion ´ de Microanalisis, ´CENTRO DE INVESTIGACION ´ TECNOLOG´IA E INNOVACION ´ (CITIUS). Universidad de Sevilla, for the use of some of the chromatographic equipment. Funding for open access charge: Universidad de Huelva / CBUA.
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026