RT Journal Article T1 Determination of antioxidant activity of spices and their active principles by Differential Pulse Voltammetry A1 Palma López, Alberto A1 Ruiz Montoya, Mercedes A1 Fernández Arteaga, Jesús A1 Rodríguez Mellado, José Miguel AB The anodic oxidation of mercury in the presence of hydrogen peroxide in differential pulse voltammetry (DPV) was used to determine the antioxidant (AO) character of radical scavengers. Hydroperoxide radical is formed at the potentials of the oxidation peak on mercury electrodes, such radical reacting with the antioxidants in different extension. The parameter C10 (antioxidant concentration at which the peak area decreases by 10%) is used to measure the scavenging activity of the individual antioxidants. To establish the scavenging activity of antioxidant mixtures as a whole, the parameter, μ10 as the reverse of V10, V10 being the volume necessary to decrease the peak area in DPV by 10%, was selected. Higher μ10 values correspond to higher scavenging activity. The studies have been extended to aqueous extracts of some species. The results may be useful in explaining the effect of spices in vitro and in vivo studies. PB American Chemical Society SN 0021-8561 SN 1520-5118 (electrónico) YR 2014 FD 2014 LK http://hdl.handle.net/10272/11400 UL http://hdl.handle.net/10272/11400 LA eng NO Palma López, A., Ruiz Montoya, M., Fernández Arteaga, J., Rodríguez Mellado, J.M.: "Determination of antioxidant activity of spices and their active principles by Differential Pulse Voltammetry". Journal of Agricultural and Food Chemistry. Vol. 62, n. 3, págs. 582–589, (2014). DOI: 10.1021/jf404578a DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026