RT Journal Article
T1 Metabolite profiling for the identification of altered metabolic pathways in Alzheimer's disease
A1 González Domínguez, Raúl
A1 García Barrera, Tamara
A1 Gómez Ariza, José Luis
AB Gas chromatography coupled to mass spectrometry is the most frequent tool for metabolomic profiling of low molecular weight metabolites. Its suitability in health survey is beyond doubt, given that primary metabolites involved in central pathways of metabolism are usually altered in diseases. The objective of this work is to investigate metabolic differences in serum between Alzheimer's disease patients and healthy controls in order to elucidate pathological mechanisms underlying to disease. Alterations in levels of 23 metabolites were detected, including increased lactic acid, α-ketoglutarate, isocitric acid, glucose, oleic acid, adenosine and cholesterol, as well as decreased urea, valine, aspartic acid, pyroglutamate, glutamine, phenylalanine, asparagine, ornithine, pipecolic acid, histidine, tyrosine, palmitic and uric acid, tryptophan, stearic acid and cystine. Metabolic pathway analysis revealed the involvement of multiple affected pathways, such as energy deficiencies, oxidative stress, hyperammonemia, and others. Moreover, it is noteworthy that some of these compounds have not been previously described in AD research, such as α-ketoglutarate, isocitrate pipecolic acid, pyroglutamate and adenosine, confirming the potential of this metabolomic approach in the search of novel potential markers for early detection of Alzheimer's disease.
PB Elsevier
SN 0731-7085
SN 1873-264X (electrónico)
YR 2015
FD 2015
LK http://hdl.handle.net/10272/14595
UL http://hdl.handle.net/10272/14595
LA eng
NO González Domínguez, R., García Barrera, T., Gómez Ariza, J.L.: "Metabolite profiling for the identification of altered metabolic pathways in Alzheimer's disease". Journal of Pharmaceutical and Biomedical Analysis. Vol. 107, págs.  75-81, (2015). DOI: 10.1016/j.jpba.2014.10.010
NO This work was supported by the Ministerio de Economia y Competitividad (Grant CTM2009CTM2012-38720-C03-01 and the Consejeria de Innovacion, Ciencia y Empresa (Junta de Andalucia) (Grants P008 FQM-3554 and P009-FQM-4659). Raul Gonzalez Dominguez thanks the Ministerio de Educacion for a predoctoral scholarship (Grant number AP2010-4278). The authors also thank to Dr. Alberto Blanco and Carlos Salgado from Hospital Juan Ramon Jimenez for providing serum samples.
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026