RT Journal Article T1 Bioactive pectic polysaccharides from bay tree pruning waste: Sequential subcritical water extraction and application in active food packaging A1 Rincón, E. A1 Espinosa, E. A1 García Domínguez, María Trinidad A1 Balu, A. M. A1 Vilaplana, F. A1 Serrano, L. A1 Jiménez Quero, A. AB The potential isolation of bio-active polysaccharides from bay tree pruning waste was studied using sequential subcritical water extraction using different time-temperature combinations. The extracted polysaccharides were highly enriched in pectins while preserving their high molecular mass (10–100 kDa), presenting ideal properties for its application as additive in food packaging. Pectin-enriched chitosan films were prepared, improving the optical properties (=95% UV-light barrier capacity), antioxidant capacity (?95% radical scavenging activity) and water vapor permeability (=14 g·Pa-1·s-1·m-1·10-7) in comparison with neat chitosan-based films. Furthermore, the antimicrobial activity of chitosan was maintained in the hybrid films. Addition of 10% of pectins improved mechanical properties, increasing the Young's modulus 12%, and the stress resistance in 51%. The application of pectin-rich fractions from bay tree pruning waste as an additive in active food packaging applications, with triple action as antioxidant, barrier, and antimicrobial has been demonstrated. PB Elsevier SN 0144-8617 YR 2021 FD 2021 LK http://hdl.handle.net/10272/20251 UL http://hdl.handle.net/10272/20251 LA eng NO Rincón, E., Espinosa, E., García-Domínguez, M. T., Balu, A. M., Vilaplana, F., Serrano, L., & Jiménez-Quero, A. (2021). Bioactive pectic polysaccharides from bay tree pruning waste: Sequential subcritical water extraction and application in active food packaging. In Carbohydrate Polymers (Vol. 272, p. 118477). Elsevier BV. https://doi.org/10.1016/j.carbpol.2021.118477 NO Authors would like to thank the Spanish Ministry of Science and Innovation (Ramon y Cajal contract RYC-2015-17109) and Universidad de Cordoba, ´ Spain (Predoctoral Grant 2019) for the financial support during this work. DS Repositorio Institucional de la Universidad de Huelva RD 31 may 2026