RT Journal Article
T1 Development of a New Method for the Absolute Quantification of Selenoproteins in Chicken Serum by Heteroatom-Tagged Proteomics
A1 Callejón Leblic, María Belén
A1 Hachemi, Mohammed A
A1 Cardoso, Denise
A1 García Barrera, Tamara
AB Selenium (Se) is an essential trace element for chickens that develops, through selenoproteins, important physiological functions. Although selenogen expression and Western blot analyses provided valuable information, there is a lack of information about the serum selenoproteome and quantitative data. In this work, we developed a new analytical method for the absolute quantification of selenoproteins and total selenometabolites in chicken serum using heteroatom-tagged proteomics, based on chromatographic separation and Se detection as a “tag” in an atomic detector. The approach was combined with bottom-up proteomics using liquid chromatography coupled to organic mass spectrometry after a tryptic digestion, and selenoprotein W was identified in chicken serum. Our results indicated that the majority of chicken serum Se is as selenoprotein P, followed by selenoprotein W, selenometabolites, glutathione peroxidase, and selenoalbumin. The approach could be a valuable tool to assess Se and selenoprotein status for the optimisation of supplementation strategies to meet the Se requirements of poultry.
PB American Chemical Society
SN 0021-8561
SN 1520-5118 (electrónico)
YR 2026
FD 2026
LK https://hdl.handle.net/10272/27975
UL https://hdl.handle.net/10272/27975
LA eng
NO Callejón-Leblic, B., Hachemi, M. A., Cardoso, D., & García-Barrera, T. (2026). Development of a New Method for the Absolute Quantification of Selenoproteins in Chicken Serum by Heteroatom-Tagged Proteomics. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/acs.jafc.5c12762
NO The authors would like to acknowledge the Project PCM_00023 “Monitorización ambiental mediante metodologías ómicas y analíticas utilizando aves y sus hábitats como bioindicadores de calidad ambiental (MONAMBIO)”, from the Andalusian Regional Government, Ministry of University, Research and Innovation, within the framework of the Complementary Plan for Marine Sciences and the Recovery, Transformation and Resilience Plan, in its call for proposals for the 2023 financial year (Measure: C17.I1), Grant 100% cofinanced by the European Union through the European Union Recovery and Resilience Facility, ″Recovery, Transformation and Resilience Plan - Funded by the European Union - NextGenerationEU″, Project TED2021-130361B−I00 (Ministry of Science and Innovation (MCIN), Knowledge Generation, MCIN/AEI/10.13039/501100011033, and European Union “NextGenerationEU”/PRTR. The authors are grateful to the FEDER Grant UNHU13-1E-161.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026