RT Journal Article
T1 BASHY Dye Platform Enables the Fluorescence Bioimaging of Myelin Debris Phagocytosis by Microglia during Demyelination
A1 Pinto, Maria V.
A1 Santos, Fabio
A1 Barros, Catarina
A1 Ribeiro, Ana Rita
A1 Pischel, Uwe
A1 Gois, Pedro
A1 Fernandes, Adelaide
AB Multiple sclerosis (MS) is a demyelinating disease of the central nervous system that is characterized by the presence of demyelinated regions with accumulated myelin lipid debris.Importantly, to allow effective remyelination, such debris must be cleared by microglia. Therefore,the study of microglial activity with sensitive tools is of great interest to better monitor the MS clinicalcourse. Using a boronic acid-based (BASHY) fluorophore, specific for nonpolar lipid aggregates,we aimed to address BASHY’s ability to label nonpolar myelin debris and image myelin clearancein the context of demyelination. Demyelinated ex vivo organotypic cultures (OCSCs) and primarymicroglia cells were immunostained to evaluate BASHY’s co-localization with myelin debris andalso to evaluate BASHY’s specificity for phagocytosing cells. Additionally, mice induced withexperimental autoimmune encephalomyelitis (EAE) were injected with BASHY and posteriorlyanalyzed to evaluate BASHY+ microglia within demyelinated lesions. Indeed, in our in vitro and exvivo studies, we showed a significant increase in BASHY labeling in demyelinated OCSCs, mostlyco-localized with Iba1-expressing amoeboid/phagocytic microglia. Most importantly, BASHY’spresence was also found within demyelinated areas of EAE mice, essentially co-localizing withlesion-associated Iba1+ cells, evidencing BASHY’s potential for the in vivo bioimaging of myelinclearance and myelin-carrying microglia in regions of active demyelination.
PB MDPI
SN 2073-4409 (electrónico)
YR 2021
FD 2021
LK https://hdl.handle.net/10272/21918
UL https://hdl.handle.net/10272/21918
LA eng
NO Pinto, M.V.; Santos, F.M.F.; Barros, C.; Ribeiro, A.R.; Pischel, U.; Gois, P.M.P.; Fernandes, A. BASHY Dye Platform Enables the Fluorescence Bioimaging of Myelin Debris Phagocytosis by Microglia during Demyelination. Cells 2021, 10, 3163. https://doi.org/10.3390/ cells10113163
NO This work was supported by Grant for Multiple Sclerosis Innovation-Merck Serono to AF, Young Investigator’s Projects for Collaborative Cross-disciplinary Studies from iMed.ULisboa to A.F. and F.S.; PTDC/QUI-QOR/29967/2017 and LISBOA-01-0145-FEDER-029967 to P.G.; the Spanish Ministry of Science, Innovation, and Universities (CTQ2017-89832-P for U.P.); the PhD grant 2021.04911.BD from Fundação para a Ciência e Tecnologia, Portugal (FCT) to M.V.P.; and in part byUIDB/04138/2020 and UIDP/04138/2020-from FCT to iMed.ULisboa.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026