RT Journal Article T1 Metabarcoding data of prokaryotes and eukaryotes inhabiting the phosphogypsum stockpiles on the salt marshes of Huelva (SW Spain) A1 Gómez Villegas, Patricia A1 Guerrero Márquez, José Luis A1 Pérez Rodríguez, Miguel A1 Bolívar Raya, Juan Pedro A1 Vigara Fernández, Javier A1 León Bañares, Rosa María AB Around 100 Mt of phosphogypsum (PG) of extreme acidity and with high concentrations of heavy metals and radionuclides have been deposited on the salt marshes of the Tinto River estuary in Huelva (SW Spain) for more than forty years. The microbial community able to thrive in these adverse conditions remains totally unknown, despite the fact that it can highly influence the biogeochemical cycle of the phospho- gypsum components and include new species with biotechnological interest. High throughput sequencing of 16S/18S rRNA encoding genes is a potent tool to uncover the microbial diversity of extreme environments. This data article de- scribes for the first time the prokaryotic and eukaryotic diversity of two water samples collected in the Huelva phosphogypsum stacks. The raw amplicons of the 16S/18S rRNA maker genes for the two phosphogypsum samples and two reference samples (seawater and the Tinto River water) obtained after sequencing on MiSeq platform are provided. The operational taxonomic units (OTUs) obtained after the treatment and clustering of the obtained reads with the QIIME2 pipeline and their taxonomic assignation performed by comparison with the SILVA database are also presented to complete the information of the article “Exploring the microbial community inhabiting the phosphogypsum stacks of Huelva (SW, Spain) by a high throughput 16S/18S rDNA Sequencing approach”. PB Elsevier YR 2022 FD 2022-02-22 LK http://hdl.handle.net/10272/21177 UL http://hdl.handle.net/10272/21177 LA eng NO Gómez-Villegas, P., Guerrero, J. L., Pérez-Rodríguez, M., Bolivar, J. P., Vigara, J., & León, R. (2022). Metabarcoding data of prokaryotes and eukaryotes inhabiting the phosphogypsum stockpiles on the salt marshes of Huelva (SW Spain). In Data in Brief (Vol. 41, p. 107989). Elsevier BV. https://doi.org/10.1016/j.dib.2022.107989 NO Artículo científico de metadatos bioinformáticos NO The authors thank Fertiberia S.A. for its support in obtaining the water samples used in thisstudy. P. Gómez-Villegas acknowledges the financial support of the University of Huelva (EPIT2016–17). This research was funded by University of Huelva and the Operative FEDER Program-Andalucía 2014-2020 (UHU-1257518 and UHU-1255876); The SUBV. COOP.ALENTEJO-ALGARVE-ANDALUCIA 2021; The European Regional Development Fund through the Agencia Estatal de In-vestigación (research grant PID 2019–110438RB-C22) and the Andalucian government (I + D + i-JA-PAIDI-Retos project 2020- PY20_00728) NO Funding for open access charge: Universidad de Huelva / CBUAFunding for open access charge: Universidad de Huelva / CBUA DS Repositorio Institucional de la Universidad de Huelva RD 31 may 2026