RT Journal Article
T1 Data-Driven Discovery of a New Fluorescent BASHY Dye for Bioimaging
A1 Ravasco, Joaõ M.J.M.
A1 Fernández Arteaga, Jesús
A1 Pischel, Uwe
A1 Gois, Pedro M. P.
AB Fluorescent molecules play a crucial role in biomedicine by facilitating the visualization and tracking of biologicalprocesses with sensitivity and specificity. However, tailoring their structure to meet the demands of live cell and in vivo imagingpresents a significant challenge due to the intricate interplay of factors governing their structural and photophysical properties. In thisstudy, we explored the potential of using multivariate linear free-energy relationships (mLFER) to optimize a multicomponentfluorescent platform. We prepared a small library of 20 fluorescent boronic-acid-derived salicylidenehydrazone (BASHY) complexesusing a versatile reaction protocol and characterized their chemical stability in water-containing media. The obtained data served asinput for the development of an mLFER model, enabling the prediction of a new BASHY dye and unraveling previously unknownmechanisms governing the stability of this unique platform of fluorescent dyes. The optimized dye was successfully employed in livecell experiments and in zebrafish larvae
PB American Chemical Society
SN 2691-3704 (electrónico)
YR 2024
FD 2024-11
LK https://hdl.handle.net/10272/24516
UL https://hdl.handle.net/10272/24516
LA eng
NO Ravasco, J. M. J. M., Felicidade, J., Pinto, M. V., Santos, F. M. F., Campos-González, R., Arteaga, J. F., Mehraz, M., Langevin, C., Fernandes, A., Nguyen, H.-C., Ng, D. Y. W., Coelho, J. A. S., Pischel, U., & Gois, P. M. P. (2024). Data-Driven Discovery of a New Fluorescent BASHY Dye for Bioimaging. In JACS Au. American Chemical Society (ACS). https://doi.org/10.1021/jacsau.4c00473
NO We are grateful for the financial support by the Fundação paraa Ciencia ̂ e a Tecnologia, Ministério da Ciencia ̂ e da Tecnologia[FCT-MCTES; 2021/04125/CEECIND for F.M.F.S., 2020/02383/CEECIND (10.54499/2020.02383.CEECIND/CP1595/CT0005) for J.A.S.C., PTDC/MED-PAT/2582/2021 for A.F., PTDC/QUI-OUT/3989/2021, UIDB/04138/2020, and UIDP/04138/2020 for the Research Institute forMedicines−iMed.ULisboa]. The NMR spectrometers are partof the National NMR Network (PTNMR) and are partiallysupported by Infrastructure Project No. 022161 (cofinancedby FEDER through COMPETE 2020, POCI, and PORL andFCT through PIDDAC). The X-ray infrastructure wasfinanced by FCT-MCTES through project RECI/BBB-BEP/0124/2012. Further we thank the Spanish Ministry of Scienceand Innovation (grant PID2020-119992GB-I00; PID2023-152556NB-I00 for U.P.) and the University of Huelva/Junta deAndalucía (UHU202070 for U.P.), both cofinanced byFEDER, for generous funding. The zebrafish experimentswere performed at the INRAE Infectiology of Fishes andRodents Facility (IERP-UE907, Jouy-en-Josas ResearchCenter, France, doi.org/10.15454/1.5572427140471238E12),which belongs to the National Research Infrastructure for theControl of Animal and Zoonotic Emerging Infectious Diseasesthrough In Vivo Investigation (EMERG’IN: doi.org/10.15454/90CK-Y371). The authors thank Magalie Bouvet and DimitriRigaudeau at IERP for zebrafish husbandry. M.N. wassupported by the animal health department of INRAE
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026