RT Journal Article
T1 Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material
A1 Lebrón, José Antonio
A1 Ostos, Francisco José
A1 López López, Manuel
A1 Moyá, María Luisa
AB Gene therapy is a therapeutic process consisting of the transport of genetic material intocells. The design and preparation of novel carriers to transport DNA is an important researchline in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixtureof lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixedwith the phospholipid DOPE. A relation between the size and the molar fraction   was foundand a multidisciplinary study about the interaction between the metallo-liposomes and DNA wasperformed. The metallo-liposomes/DNA association was quantified and a relationship betweenKapp and   was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scatteringand agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structureof the liposomes. L/D values corresponding to the polynucleotide’s condensation were estimated.In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells thanfor cancer cell lines, and a good internalization into cells. The latter as well as the transfectionmeasurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability ofthe Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.
PB MDPI
SN 1999-4923
YR 2020
FD 2020-05
LK http://hdl.handle.net/10272/18268
UL http://hdl.handle.net/10272/18268
LA eng
NO Lebrón, J. A., Ostos, F. J., López López, M., ... Moyá, M. L. (2020). Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material. Pharmaceutics, 12(5), 482. DOI: https://doi.org/10.3390/pharmaceutics12050482
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jul 2026