RT Journal Article
T1 Antifungal Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from Allium cepa against Verticillium dahliae: In Vitro and in Planta Assays
A1 Falcón Piñeiro, Ana
A1 Remesal, Efrén
A1 Noguera Manzano, Miguel
A1 Ariza, Juan José
A1 Guillamón, Enrique
A1 Baños, Alberto
A1 Juan Antonio Navas-Cortes, 
AB Verticillium wilt, caused by Verticillium dahliae, is the most devastating soil-borne fungaldisease of olive trees worldwide. Currently, there is no effective measure available to control thepathogen in diseased plants in open field conditions. Searching more effective and sustainablesolutions are a priority for the olive sector. The existing alternatives for disease control include theuse of biological control microorganisms and compounds of natural origin from plants, such asAlliaceae. Propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are twoorganosulfur compounds derived from Allium cepa with a widely documented antimicrobial activity.The aim of this study was to evaluate the antifungal activity of PTS and PTSO against the defoliatingand non-defoliating V. dahliae pathotypes. Firstly, several in vitro tests were performed (MinimumAntifungal Concentration, susceptibility studies according to the Kirby–Bauer disk-diffusion method,antifungal activity through aerial diffusion and effect on mycelial growth). The ability of bothcompounds to sanitize soil was evaluated using a sterile substrate inoculated with V. dahliae. Finally,challenges in growth chambers were carried out. PTS and PTSO generated growth inhibition zones inagar diffusion and the gas phase, and the mycelial growth of all the V. dahliae strains was significantlyaltered. The V. dahliae population in soil was considerably reduced after the sanitization. Finally,in planta assays demonstrated the ability of these compounds to reduce disease related parametersand their contribution to control the phytopathogen. In conclusion, the results showed that the PTSand PTSO from Allium cepa display in vitro and in vivo antifungal activity against V. dahliae andsuggested that both compounds could be used as natural and environmentally friendly tools forVerticillium wilt managemen
PB MDPI
SN 2309-608X (electrónico)
YR 2021
FD 2021
LK http://hdl.handle.net/10272/20268
UL http://hdl.handle.net/10272/20268
LA eng
NO Falcón-Piñeiro, A., Remesal, E., Noguera, M., Ariza, J. J., Guillamón, E., Baños, A., & Navas-Cortes, J. A. (2021). Antifungal Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from Allium cepa against Verticillium dahliae: In Vitro and in Planta Assays. In Journal of Fungi (Vol. 7, Issue 9, p. 736). MDPI AG. https://doi.org/10.3390/jof7090736
NO This research has been carried out within the project GRUPO OPERATIVO SALUDOLIVAR from the Spanish Rural Development Program (2014–2020) funded by the Spanish Ministryof Agriculture, Fisheries and Food and co-financed by 80% by the European Agricultural Fund forRural Development (FEADER) (Total investment 384.830,96 EUR). This study is part of the IndustrialDoctorate of the doctoral student Ana Falcón Piñeiro, granted by the State Research Agency of theSpanish Government, with the following reference: DIN2019-010792We would also like to acknowledge the work of Jose Manuel García-Madero inediting the text
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026