RT Journal Article T1 Improvements in the cultivation of Botryococcus braunii using commercial fertilisers A1 Bermejo, Elisabeth A1 González, Cristina A1 Montero Lobato, Zaida A1 Vílchez Lobato, Carlos A1 Garbayo Nores, Inés A1 Cuaresma Franco, María AB Agricultural fertilisers (NPKs) have been recognised as an alternative to make microalgae cultivation cheaper as well as simpler in terms of the preparation of the medium. Botryococcus braunii, a green microalga, has the almost unique capacity to accumulate and excrete large amounts of long-chain hydrocarbons and/or interesting groups of polysaccharides which can be further converted into bio-products. However, limitations in growth are currently hindering its industrial production. In this work, the use of different agricultural fertilisers (NPKs) was evaluated for the cultivation of two B. braunii races (A and B) in terms of productivity and final media labour and cost. Results corroborated that fertilisers-based media are easier to prepare and their prices are considerably lower compared to common culture media. At the same time, good growth performance and photosynthetic efficiency can be maintained and carbohydrate and hydrocarbon productivities can be further enhanced. However, special attention should be given to each particular strain since different behaviour in growth and metabolite production can be observed depending on the media composition. The significantly higher productivities obtained, together with the important reduction in media price when using commercial fertilisers and the advantages related to the easiness to prepare the culture media based on NPK fertilizers, represent an important achievement for the development of an industry based on these renewable products SN 1573-5176 YR 2021 FD 2021-05-14 LK https://hdl.handle.net/10272/24957 UL https://hdl.handle.net/10272/24957 LA eng NO Bermejo, E., González, C., Montero, Z., Vílchez, C., Garbayo, I., Cuaresma, M. (2021) Improvements in the cultivation of Botryococcus braunii using commercial fertilisers. Journal of Applied Phycology 33: 1939-1948 NO This work has been supported financially by the European Union under the Seventh framework programme (ProjectSPLASH: http://www. eu-splash.eu; contract number 311956). DS Repositorio Institucional de la Universidad de Huelva RD 1 jun 2026