@article{10272/24952,
year = {2020},
month = {9},
url = {https://hdl.handle.net/10272/24952},
abstract = {Current interest in the production of commercial biopolymers which are derived from renewable resources arises from the necessity tomove from a fossil-based to a more sustainable economy. Microalgae are considered potential biopolymer producers although their use at an industrial scale is not feasible yet. In this sense, medium optimisation and operational conditions can be considered the first steps leading to growth and product productivity enhancement which are a prerequisite to boost the microalgae industry. This manuscript describes medium optimisation for two promising strains of the green alga Botryococcus braunii, B. braunii CCALA-778 (race A) and B. braunii AC761 (race B), which are natural producers of (exo)polysaccharides and hydrocarbons of high interest in the biopolymers industry. Medium optimisation was based on a three-step research process: (i) a literature survey to identify macro- and micronutrients that could be limiting Botryococcus growth, (ii) a 3-level factorial design experiment in microwell plates to evaluate modified media accordingly and (iii) validation of previous results in batch cultivation in Roux flasks. The results obtained show the significant impact of culturemedia optimisation in the growth dynamics and valuable compound accumulation of B. braunii. Attention should be given to each particular Botryococcus race and media.
should be defined accordingly. But overall, the new optimised media resulted in higher productivities which may contribute to
reach sustainability of Botryococcus cultivation.},
organization = {This work has been supported financially by the European Union under the Seventh framework programme (Project
SPLASH: http://www.eu-splash.eu; contract number 311956).},
publisher = {Springer},
title = {Medium optimisation as a first step towards the feasible production of biopolymers with Botryococcus braunii},
doi = {https://doi.org/10.1007/s10811-020-02245-7},
author = {Vílchez Lobato, Carlos and Bermejo, Elisabeth and Muñoz, Ángel and Ramos Merchante, Adrián and Garbayo Nores, Inés and Cuaresma Franco, María},
}