RT Journal Article
T1 Medium optimisation as a first step towards the feasible production of biopolymers with Botryococcus braunii
A1 Vílchez Lobato, Carlos
A1 Bermejo, Elisabeth
A1 Muñoz, Ángel
A1 Ramos Merchante, Adrián
A1 Garbayo Nores, Inés
A1 Cuaresma Franco, María
AB Current interest in the production of commercial biopolymers which are derived from renewable resources arises from the necessity tomove from a fossil-based to a more sustainable economy. Microalgae are considered potential biopolymer producers although their use at an industrial scale is not feasible yet. In this sense, medium optimisation and operational conditions can be considered the first steps leading to growth and product productivity enhancement which are a prerequisite to boost the microalgae industry. This manuscript describes medium optimisation for two promising strains of the green alga Botryococcus braunii, B. braunii CCALA-778 (race A) and B. braunii AC761 (race B), which are natural producers of (exo)polysaccharides and hydrocarbons of high interest in the biopolymers industry. Medium optimisation was based on a three-step research process: (i) a literature survey to identify macro- and micronutrients that could be limiting Botryococcus growth, (ii) a 3-level factorial design experiment in microwell plates to evaluate modified media accordingly and (iii) validation of previous results in batch cultivation in Roux flasks. The results obtained show the significant impact of culturemedia optimisation in the growth dynamics and valuable compound accumulation of B. braunii. Attention should be given to each particular Botryococcus race and media.should be defined accordingly. But overall, the new optimised media resulted in higher productivities which may contribute toreach sustainability of Botryococcus cultivation.
PB Springer
SN 0921-8971 (electrónico)
YR 2020
FD 2020-09
LK https://hdl.handle.net/10272/24952
UL https://hdl.handle.net/10272/24952
LA eng
NO Bermejo, E., Muñoz, A., Ramos-Merchante, A., Vílchez, C., Garbayo, I., Cuaresma, M. (2020) Medium optimisation as a first step towards the feasible production of biopolymers with Botryococcus braunii. Journal of Applied Phycology 32: 3667-3678
NO This work has been supported financially by the European Union under the Seventh framework programme (ProjectSPLASH: http://www.eu-splash.eu; contract number 311956).
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026