@article{10272/23086,
year = {2024},
url = {https://hdl.handle.net/10272/23086},
abstract = {This study aimed at optimizing the accumulation of phytoene in Chlorella sorokiniana by using norflurazon and investigating the capacity of green and traditional solvents to extract carotenoids by ultrasound-assisted extraction with and without previous milling.
Phytoene-rich first described C. sorokiniana biomass was used, both fresh, freeze-dried, and encapsulated. The ideal dose of norflurazon (1 µg/mL) was selected to block the carotenoid pathway at the level of phytoene desaturase and induce the accumulation of phytoene in C. sorokiniana. A mill pre-treatment allowed a higher recovery of carotenoids compared to non-milled samples, in both the freeze-dried and encapsulated matrices. 2-Methyloxolane provided a higher total carotenoid content (4.75–5546.96 µg/g) compared to the other solvents tested in all the matrices, proving a promising bio-based solvent to replace traditional organic ones for the extraction of microalgal carotenoids.},
organization = {This study was financially supported by grants PID2019-110438RB- C21 (NEWCARFOODS) and PID2019-110438RB-C22 (BESTALGAE4CAROT), funded by MCIN/AEI/ 10.13039/501100011033. PMB was supported by a posdoc fellowship from Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía (PAIDI 2020). AMO was supported by a grant associated to grant PID2019-110438RB-C21 (NEWCARFOODS). A.L-V also thanks Next Generation European Fundsand theMinistry of Universities of Spainfor funding the Recualificación del Profesorado Universitario system. AMO, PMB, AMBG, and AJMM are members of the Spanish Carotenoid Network (CaRed), grant RED2022-134577-T, funded by MCIN/AEI/10.13039/501100011033.},
publisher = {Elsevier},
title = {Ultrasound-assisted extraction of carotenoids from phytoene-accumulating Chlorella sorokiniana microalgae: Effect of milling and performance of the green biosolvents 2-methyltetrahydrofuran and ethyl lactate},
doi = {10.1016/j.foodchem.2023.137437},
author = {Morón Ortiz, Ángeles and Mapelli Brahm, Paula and León Vaz, Antonio and Benítez González, Ana M. and León Bañares, Rosa María and Meléndez Martínez, Antonio J.},
}