RT Journal Article T1 Differentiation of Lesueurigobius sanzi and L. suerii through otolith morphometric analysis A1 Rubiales Gómez, Jaime S. A1 Castro Gutiérrez, Jairo A1 Rodríguez García, Carlos A1 Sarmiento Carbajal, Jésica A1 Cabrera Castro, Remedios AB Otolith morphology is a valuable tool for species identification, particularly for morphologically similar species. This study evaluates the potential of otolith morphometry to distinguish Lesueurigobius sanzi and L. suerii, two closely related species commonly found as fishery discards in the Gulf of Cádiz. A total of 1,019 individuals (L. sanzi: 921, L. suerii: 98) were collected and their otoliths were analysed using the R package "shapeR" to obtain morphometric parameters and standardised wavelet coefficients. Statistical analyses revealed significant morphological differences between the species. Principal Component Analysis (PCA) showed that otolith length, perimeter, and shape indices contribute significantly to species differentiation. Furthermore, a Linear Discriminant Analysis (LDA) based on standardised wavelet coefficients demonstrated high classification accuracy (91.6%), confirming the effectiveness of this approach for species identification. These findings highlight the reliability of otolith morphometry as a robust method for distinguishing L. sanzi and L. suerii, providing valuable insights for fisheries management and biodiversity studies. This research contributes to the development of classification methods, essential for sustainable fishery assessments and ecological research. PB Elsevier SN 1096-0015 YR 2026 FD 2026 LK https://hdl.handle.net/10272/28128 UL https://hdl.handle.net/10272/28128 LA eng NO Rubiales-Gómez, J. S., Castro-Gutiérrez, J., Rodríguez-García, C., Sarmiento-Carbajal, J., & Cabrera-Castro, R. (2026). Differentiation of Lesueurigobius sanzi and L. suerii through otolith morphometric analysis. Estuarine, Coastal and Shelf Science, 332, 109763. https://doi.org/10.1016/j.ecss.2026.109763 NO This research has been carried out within the framework of the ECOFISH project: eco-innovative strategies for sustainable fishing in the Gulf of Cadiz SPA. This initiative has been supported by the Biodiversity Foundation, the Ministry for Ecological Transition and Demographic Challenge, through the Pleamar Program, co-financed by the European Maritime and Fisheries Fund (EMFF) (grant numbers: 2019-016/PV/ PLEAMAR18/PT; 2020-013/PV/PLEAMAR19/PT; 2020-055/PV/ PLEAMAR20/PT; 2021/PV/PLEAMAR20-21/PT; 2021-060/PV/PLEA MAR21/PT). DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026