RT Journal Article
T1 Characterization of PLGA nanoparticles for stabilization and controlled release of antimicrobial peptides into marine bivalves
T2 Caracterización de nanopartículas de PLGA para la estabilización y liberación controlada de péptidos antimicrobianos en bivalvos marinos
A1 Molina Márquez, Ana
A1 López López, Manuel
A1 Rengel, Rocío
A1 López Sanmartín, Monserrat
A1 Domínguez, Elena
A1 Pereira Jaramillo, Rocío
A1 López Cornejo, Pilar
A1 Lebrón, José Antonio
A1 Perazzoli, Gloria
A1 Carbajo Cobacho, José
A1 León Bañares, Rosa María
K1 Antimicrobial peptides
K1 Bivalves
K1 Clams
K1 Caerin
K1 PLGA
K1 Encapsulation
AB Nanoparticles (NPs) have revolutionized the field of medicine and veterinary for both detection and treatment of infection diseases, however, their utilization to supply drugs or bioactive metabolites to marine invertebrates has been insufficiently investigated. Antimicrobial peptides (AMPs) have gained increasing attention as potential alternatives to overcome the shortcomings associated with conventional therapeutic strategies. Using an emulsion/evaporation approach, spherical poly(lactic-co-glycolic acid) (PLGA) NPs charged with D-Caerin (D-CAE) peptide have been prepared. The obtained NPs showed a bimodal size distribution, with mean diameters of 130 ± 4 and 421 ± 19 nm. Among the tested formulation, NPs with a peptide/polymer ratio of 0.02 exhibited the best physico-chemical properties, achieving encapsulation efficiencies of 70 %. Release studies demonstrated that 73.5 % of the peptide was liberated from loaded the NPs within 40 h. Furthermore, the D-CAE peptide retained its antimicrobial activity following encapsulation in PLGA NPs and exhibited bactericidal effects against two prevalent pathogens in aquaculture species. Finally, it has been shown that peptide-loaded NPs are effectively ingested by clam post-larvae, suggesting the potential of peptide-PLGA NPs as biocompatible nanocarriers for delivery of AMPs into bivalves and other invertebrates.
PB Elsevier
YR 2025
FD 2025-10-22
LK https://hdl.handle.net/10272/28203
UL https://hdl.handle.net/10272/28203
LA eng
NO Ana Molina-Márquez, Manuel López-López, Rocío Rengel, Monserrat López-Sanmartín, Elena Domínguez, Rocío Pereira-Jaramillo, Pilar López-Cornejo, José Antonio Lebrón, Gloria Perazzoli, José Carbajo-Cobacho, Rosa León. Characterization of PLGA nanoparticles for stabilization and controlled release of antimicrobial peptides into marine bivalves, Aquaculture, Volume 613, Part 1, 2026, 743334,https://doi.org/10.1016/j.aquaculture.2025.743334
NO This research was financially supported by grant PID2022- 140995OB-C21 (CARACAS), funded by MCIN/AEI/ 10.130 39/501100011033; and Consejería de Educacion ´ y Ciencia de la Junta de Andalucía- USE (FQM-206).
NO Funding for open access charges: Universidad de Huelva/CBUA
DS Repositorio Institucional de la Universidad de Huelva
RD 28 may 2026