RT Journal Article
T1 Metabolomics insights into the shared and cultivar-related mechanisms controlling postharvest cold tolerance in Cucurbita pepo L
A1 García Fuentes, Alicia
A1 Castro Cegrí, Alejandro
A1 García Pérez, Pascual
A1 Palma Martín, Francisco José
A1 Martínez Martínez, Cecilia
A1 Garrido Garrido, María Dolores
A1 Jamilena, Manuel
A1 Lucini, Luigi
AB Zucchini (Cucurbita pepo L.) is highly sensitive to chilling injury during cold storage, which leads to surface damage, quality loss, and reduced nutraceutical value. Fruits were stored at 4 ◦C, and samples were collected at harvest (T0), 3 days (T3), and 14 days (T14) of cold storage. To elucidate the biochemical determinants of cold tolerance, we selected five cold-tolerant and five cold-sensitive cultivars from a previous screening of 126 accessions based on differences in chilling injury index (CI), weight loss (WL), and physiological indicators of oxidative stress. Metabolomic profiling was performed on fruit  exocarp tissue using an untargeted LC–MS approach. Despite genotypic variability, all cultivars displayed a shared metabolic response to cold storage characterized by the accumulation of N6,N6,N6-trimethyl-L-lysine and 6-hydroxymethyl-7,8-dihydropterin, intermediates in carnitine and folate biosynthesis. Notably, tolerant cultivars maintained higher levels of these metabolites throughout storage, suggesting the presence of a metabolic signature associated with improved cold tolerance. Beyond this shared response, tolerant cultivars exhibited distinct metabolic adjustments involving nucleotides, phenolic compounds, amino acids, phytohormones, vitamins, and cucurbitacins. Overall, these results provide new insights into metabolic responses associated with postharvest cold tolerance in zucchini and highlight candidate metabolic markers that may be useful for breeding programs aimed at improving postharvest performance and shelf life.
PB Elsevier
SN 0304-4238
SN 1879-1018 (electrónico)
YR 2026
FD 2026
LK https://hdl.handle.net/10272/28445
UL https://hdl.handle.net/10272/28445
LA eng
NO García, A., Castro-Cegrí, A., García-Pérez, P., Palma, F., Martínez, C., Garrido, D., Jamilena, M., & Lucini, L. (2026). Metabolomics insights into the shared and cultivar-related mechanisms controlling postharvest cold tolerance in Cucurbita pepo L. Scientia Horticulturae, 359, 114766. https://doi.org/10.1016/j.scienta.2026.114766
NO This work was supported by research grant PID2020-118080RB-C21 at UAL and PID2020-118080RB-C22 at UGR, funded by ‘Ministerio de Ciencia, Innovación y Universidades’ together with EU FEDER funds. Pascual García-Pérez thanks the financial support through the Ramón y Cajal program (reference: RYC2023-044123-I) by the Spanish Ministry of Science, Innovation and Universities, the National Research Agency (MCIU/AEI/10.13039/501100011033) and the European Social Fund Plus (FSE+).
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026