RT Journal Article
T1 Immunotoxicity of polystyrene nanoplastics in different hemocyte subpopulations of Mytilus galloprovincialis
A1 Sendra, Marta
A1 Carrasco Braganza, María
A1 Yeste, Pilar María
A1 Vila Spinola, Marta
A1 Blasco Moreno, Julián
AB Plastic represents 60-80% of litter in the ocean. Degradation of plastic to small fragments leads to the formation of microplastics (MPs <5 mm) and nanoplastics (NPs <1 µm). One of the most widely used and representative plastics found in the ocean is polystyrene (PS). Among marine organisms, the immune system of bivalves is recognized as suitable to assess nanomaterial toxicity. Hemocyte subpopulations [R1 (large granular cells), R2 (small semi-granular cells) and R3 (small agranular or hyaline cells)] of Mytilus galloprovincialis are specialized in particular tasks and functions. The authors propose to examine the effects of different sizes (50 nm, 100 nm and 1 μm) PS NPs on the different immune cells of mussels when they were exposed to (1 and 10 mg·L−1) of PS NPs. The most noteworthy results found in this work are: (i) 1 µm PS NPs provoked higher immunological responses with respect to 50 and 100 nm PS NPs, possibly related to the higher stability in size and shape in hemolymph serum, (ii) the R1 subpopulation was the most affected with respect to R2 and R3 concerning immunological responses and (iii) an increase in the release of toxic radicals, apoptotic signals, tracking of lysosomes and a decrease in phagocytic activity was found in R1.
PB Nature Research
SN 2045-2322 (electrónico)
YR 2020
FD 2020
LK http://hdl.handle.net/10272/20830
UL http://hdl.handle.net/10272/20830
LA eng
NO Sendra, M., Carrasco-Braganza, M.I., Yeste, P.M. et al. Immunotoxicity of polystyrene nanoplastics in different hemocyte subpopulations of Mytilus galloprovincialis. Sci Rep 10, 8637 (2020). https://doi.org/10.1038/s41598-020-65596-8
NO Marta Sendra, Pilar Yeste and Marta Vila are grateful to CEI·MAR for their support through CEIJ-C06.1,CEIJ-C06.2, CEIJ-C06.3 projects. This research has been funded by the Spanish National Research PlanMINECO (CTM2016-75908-R). The authors are grateful to the SC-ICYT of Cádiz University (UCA) andCatalina Fernandez from IFAPA for the use of equipment to characterize NPs. We are also grateful to Alba Vergesand Ana Bartual for her support with the confocal images and images through flow cytometry with Amnis®flow cytometer (INMAR-UCA) respectively. MS also wants to acknowledge the Spanish Ministerio de Ciencia,Innovación y Universidades for her Juan de la Cierva contract (TJFI-2017-32493).
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026