RT Journal Article
T1 Multielemental fractionation in pine nuts (Pinus pinea) from different geographic origins by size-exclusion chromatography with UV and inductively coupled plasma mass spectrometry detection
A1 Gómez Ariza, José Luis
A1 Arias Borrego, Ana
A1 García Barrera, Tamara
AB Pine nuts (Pinus pinea) from different geographical origin in Spain and Portugal have been investigated concerning total element content andmetal-biomolecules size distribution patterns Mn, Zn, Ni and Cu. All the studied metals were at the highest concentration in pine nuts fromFaro and at the lowest from Catalu˜na. The most abundant element in samples was Mn at concentrations in the range of 26  g g−1 (Catalu˜na) to559 g g−1 (Faro). Zn was also present at high concentration in samples, from 25  g g−1 (Catalu˜na) to 113  g g−1 (Faro). To a deeper insightto obtain classification rules for samples, pine nuts were analyzed by size-exclusion chromatography (SEC) with UV detection and inductivelycoupled plasma mass spectrometry (ICP-MS). Two columns were used covering the molecular weigh range from <10 to 70 kDa that allowed thediscrimination of the studied samples. Data reveal that the most differential UV-profile with low molecular weight (LMW) column was obtainedwith pine nuts from Huelva. This column allows good discrimination in the range of 2126–1352 Da in which a lot of peaks can be used todifferentiate samples. The UV profiles obtained with the high molecular weight (HMW) column allows a poorer differentiation of samples, butpine nuts from Huelva, Castilla and Madrid are clearly distinguished to the others. In relation to fractionation patterns of metals, Mn allows a gooddiscrimination between samples (LMW column), Cu was the only one associated to fractions at MW> 70 kDa in sample from C´adiz, and profilesof Ni and Zn are clearly different in terms of abundance of peaks. All these chromatographic profiles for elements give valuable information aboutthe geographical origin of the studied samples and the differences found are discussed in this work
PB Elsevier
SN 0021-9673
SN 1873-3778 (electrónico)
YR 2006
FD 2006
LK https://hdl.handle.net/10272/22965
UL https://hdl.handle.net/10272/22965
LA eng
NO Gómez-Ariza, J. L., Arias-Borrego, A., & García-Barrera, T. (2006). Multielemental fractionation in pine nuts (Pinus pinea) from different geographic origins by size-exclusion chromatography with UV and inductively coupled plasma mass spectrometry detection. In Journal of Chromatography A (Vol. 1121, Issue 2, pp. 191–199). Elsevier BV. https://doi.org/10.1016/j.chroma.2006.04.025
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jun 2026