@article{10272/16424,
year = {2019},
month = {2},
url = {http://hdl.handle.net/10272/16424},
abstract = {Cefepime is an antibioticwith a broad spectrumof antimicrobial activity. However, this antibiotic
has several side effects and a high degradation rate. For this reason, the preparation and characterization
of new liposomes that are able to encapsulate this antibiotic seemto be an important research line in the
pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic
structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium.
Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with
phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE).
The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the
encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes
with phosphatidylcholinewasmeasured. The inhibitory zone in an agar plate for free cefepimewas similar
to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in
working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All
obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can
be used in bacterial infections against Escherichia coli with high inhibitory activity.},
organization = {This research was funded by the Consejeria de Educacion y Ciencia de la Junta de Andalucia (Proyecto de Excelencia P12-FQM-1105, FQM-206 and FQM-274), the VI Plan Propio Universidad de Sevilla (2018/500), and the European Union (Feder Funds).},
publisher = {MDPI},
title = {Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes},
doi = {10.3390/pharmaceutics11020069},
author = {Moyá, María Luisa and López López, Manuel and Lebrón, José Antonio and Ostos, Francisco José},
}