RT Journal Article
T1 Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
A1 Moyá, María Luisa
A1 López López, Manuel
A1 Lebrón, José Antonio
A1 Ostos, Francisco José
AB Cefepime is an antibioticwith a broad spectrumof antimicrobial activity. However, this antibiotichas several side effects and a high degradation rate. For this reason, the preparation and characterizationof new liposomes that are able to encapsulate this antibiotic seemto be an important research line in thepharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationicstructures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium.Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes withphosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE).The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased theencapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomeswith phosphatidylcholinewasmeasured. The inhibitory zone in an agar plate for free cefepimewas similarto that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured inworking conditions. The liposome without any antibiotic exerted no influence in such a rate constant. Allobtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that canbe used in bacterial infections against Escherichia coli with high inhibitory activity.
PB MDPI
SN 1999-4923
YR 2019
FD 2019-02
LK http://hdl.handle.net/10272/16424
UL http://hdl.handle.net/10272/16424
LA eng
NO Moyá, M. L., López López, M., Lebrón, J. A., ... Ostos, F. J., (2019). Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes. Pharmaceutics, 11(2), 69. DOI: https://doi.org/10.3390/pharmaceutics11020069
NO This research was funded by the Consejeria de Educacion y Ciencia de la Junta de Andalucia (Proyecto de Excelencia P12-FQM-1105, FQM-206 and FQM-274), the VI Plan Propio Universidad de Sevilla (2018/500), and the European Union (Feder Funds).
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jun 2026