RT Journal Article
T1 Arsenic metabolites in human serum and urine after seafood (Anemonia sulcata) consumption and bioaccessibility assessment using liquid chromatography coupled to inorganic and organic mass spectrometry
A1 Contreras Acuña, Manuel
A1 García Barrera, Tamara
A1 García Sevillano, Miguel Ángel
A1 Gómez Ariza, José Luis
AB The present paper reports for the first time the bioaccessibility of arsenic species in Anemonia sulcata and its metabolization in the human body after the ingestion of this seafood, a delicacy highly appreciated in the south of Spain. Speciation in tissue extracts and human fluids was carried out by anion/cation exchange chromatography with inductively coupled plasma mass spectrometry ((IC)-ICP-MS). Results obtained show that about 54% of the arsenic present in the anemones is lost after cooking, around 95% of total arsenic in cooked anemones is bioaccessible and 85% of the amount ingested is eliminated from the body by urine in 90 h. The relative abundance of As in urine varies along the time, but after the first 10 h follows the order arsenobetaine (AB) ˜ DMAV (dimethylarsinate) > arsenocholine (AsC) > tetra-methyl-arsonium (TETRA) > trimethyl-arsine oxide (TMAO) > MAV (monomethylarsonate) > iAsV > dimethylarsenoethanol (DMAE), while the major species in cooked anemones is AB followed by DMAV > dimethylmonothioarsinic acid (DMASV) > AsC > TETRA > TMAO > iAsV > glycerylphosphorylarsenocholine(GPAsC) > MAV > DMA. After 30 min from the ingestion, DMAV is the most abundant in human serum, followed by AB, MAV and AsC. Finally, the use of organic mass spectrometry allowed the standardless identification of AsC, GPAsC, DMASV and DMAE.
PB Elsevier
SN 0026-265X
YR 2014
FD 2014
LK http://hdl.handle.net/10272/14730
UL http://hdl.handle.net/10272/14730
LA eng
NO Contreras-Acuña, M., García-Barrera, T., García-Sevillano, M. A., & Gómez-Ariza, J. L. (2014). Arsenic metabolites in human serum and urine after seafood (Anemonia sulcata) consumption and bioaccessibility assessment using liquid chromatography coupled to inorganic and organic mass spectrometry. Microchemical Journal, 112, 56-64. https://doi.org/10.1016/j.microc.2013.09.007
NO The authors thank the Spanish Ministry of Science and Innovation project CTM2009-12858-C02-01 and the projects P08-FQM-03554 and P09-FQM-4659 from Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government) for funding. The authors also thank Dr. Vicenta Devesa and Dinoraz Velez (Instituto de Agroquimica y Tecnologia de Alimentos (IATA-CSIC), Valencia, Spain) and Dr. Riansares Munoz (Universidad Complutense de Madrid) for the kind donation of TMAO, TETRA and DMAS. M.A. Garcia Sevillano thanks the Ministerio de Educacion for a PhD scholarship.
DS Repositorio Institucional de la Universidad de Huelva
RD 28 may 2026