RT Journal Article
T1 Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
A1 Lebrón, José Antonio
A1 López Cornejo, Pilar
A1 García Dionisio, Elena
A1 López López, Manuel
AB The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactantswith different functional groups at the end of hydrophobic tail was studied with the goalof investigating the influence of the functional group nature on surfactant DNA compacting efficiency.The surfactants investigated were dodecyltriethylammonium bromide (DTEABr), triethyl(1-phenoxydodecyl)ammonium bromide (12PhBr), triethyl(2-naphthoxydodecyl)ammonium bromide(12NBr) and 11-(isonicotinoyloxy)-N,N,N-triethyl-1-undecanaminium bromide (11PyBr). Resultsmade evident that the surfactants’ tendencies to self-aggregation is the key factor determining theirefficiency to compact the nucleic acid. Subsequently, DOPE/12NBr/pEGFP-C1 lipoplexes, withdifferent cationic surfactant molar fractions ( ) and mass ratios (L/D), were prepared and characterized.DOPE is a zwitterionic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, andthe plasmid pEGFP-C1 carries a GFP coding sequence with the necessary regulatory elements forconstitutive expression of the gene in human cells. 12NBr was chosen because it was the most efficientDNA compacting agent among the surfactants investigated. Finally, the cytotoxicity and transfectionefficiency (TE) of DOPE/12NBr/pDNA lipoplexes, with different compositions, were investigated.
PB MDPI
SN 1999-4923
YR 2021
FD 2021-04
LK http://hdl.handle.net/10272/19893
UL http://hdl.handle.net/10272/19893
LA eng
NO Lebrón, J. A., López Cornejo, P., García Dionisio, E., ... López-López, M. (2021). Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency. Pharmaceutics, 13(4), 589. DOI: https://doi.org/10.3390/pharmaceutics13040589
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026