RT Journal Article T1 Optimization of Laccase/Mediator System (LMS) Stage Applied in Fractionation of Eucalyptus globulus A1 Loaiza Rodríguez, Javier Mauricio A1 Alfaro Martínez, Ascensión A1 López Baldovín, Francisco A1 García Domínguez, María Trinidad A1 García Domínguez, Juan Carlos AB In a biorefinery framework, a laccase/mediator system treatment following autohydrolysis was carried out for eucalyptus wood prior to soda-anthraquinone pulping. The enzymatic and autohydrolysis conditions, with a view to maximizing the extraction of hemicelluloses while preserving the integrity of glucan, were optimized. Secondly, pulping of solid phase from Eucalyptus globulus wood autohydrolysis and the enzymatic process was carried out and compared with a conventional soda-anthraquinone (AQ) pulping process. The prehydrolysis and enzymatic delignification of the raw material prior to the delignification with soda- Anthraquinone (AQ) results in paper sheets with a lower kappa number and brightness and strength properties close to conventional soda-AQ paper and a liquid fraction rich in hemicellulose compounds that can be used in additional ways. The advantage of this biorefinery scheme is that it requires a lower concentration of chemical reagents, and lower operating times and temperature in the alkaline delignification stage, which represents an economic and environmental improvement over the conventional process. PB MDPI SN 2073-4360 YR 2019 FD 2019 LK http://hdl.handle.net/10272/16315 UL http://hdl.handle.net/10272/16315 LA eng NO Loaiza, J. M., Alfaro, A., López, F., García, M. T., & García, J. C. (2019). Optimization of Laccase/Mediator System (LMS) Stage Applied in Fractionation of Eucalyptus globulus. Polymers, 11(4), 731. https://doi.org/10.3390/polym11040731 NO The authors gratefully acknowledge the funding of this work from the Andalusian Regional Ministryof Economy, Innovation, Science, and Employment (Project number RNM 2323 and FPI grant), the Ministry ofEconomy and Competitiveness, and the National Programme for Research Aimed at the Challenges of Societyand co-financed with FEDER funds, (CTQ2013-46804-C2-1-R and CTQ2017-85251-C2-1-R). DS Repositorio Institucional de la Universidad de Huelva RD 1 jun 2026