RT Journal Article
T1 Three Genes Involved in Different Signaling Pathways, carS, wcoA, and acyA, Participate in the Regulation of Fusarin Biosynthesis in Fusarium fujikuroi
A1 Díaz Sánchez, Violeta
A1 Castrillo Jiménez, Marta
A1 García Martínez, Jorge
A1 Ávalos Cordero, Francisco Javier
A1 Limón Mirón, María Carmen
AB The phytopathogenic fungus Fusarium fujikuroi has a rich secondary metabolism which includes the synthesis of very different metabolites in response to diverse environmental cues, such as light or nitrogen. Here, we focused our attention on fusarins, a class of mycotoxins whose synthesis is downregulated by nitrogen starvation. Previous data showed that mutants of genes involved in carotenoid regulation (carS, encoding a RING finger protein repressor), light detection (wcoA, White Collar photoreceptor), and cAMP signaling (AcyA, adenylate cyclase) affect the synthesis of different metabolites. We studied the effect of these mutations on fusarin production and the expression of the fus1 gene, which encodes the key polyketide synthase of the pathway. We found that the three proteins are positive regulators of fusarin synthesis, especially WcoA and AcyA, linking light regulation to cAMP signaling. Genes for two other photoreceptors, the cryptochrome CryD and the Vivid flavoprotein VvdA, were not involved in fusarin regulation. In most cases, there was a correspondence between fusarin production and fus1 mRNA, indicating that regulation is mainly exerted at the transcriptional level. We conclude that fusarin synthesis is subject to a complex control involving regulators from different signaling pathways.
PB MDPI
SN 2309-608X (electrónico)
YR 2024
FD 2024-03
LK https://hdl.handle.net/10272/23705
UL https://hdl.handle.net/10272/23705
LA eng
NO Díaz-Sánchez, V., Castrillo, M., García-Martínez, J., Avalos, J., & Limón, M. C. (2024). Three Genes Involved in Different Signaling Pathways, carS, wcoA, and acyA, Participate in the Regulation of Fusarin Biosynthesis in Fusarium fujikuroi. In Journal of Fungi (Vol. 10, Issue 3, p. 203). MDPI AG. https://doi.org/10.3390/jof10030203
NO V.D.-S. received a grant from Plan Propio de la Universidad de Sevilla. M.C. and J.G.-M.received grants funded by the Spanish Ministry of Science and Innovation. This work was fundedby projects BIO2009-11131 and PID2022-140414NB-I00 from Ministerio de Ciencia e Innovación(MCIN)/Agencia Estatal de Investigación (AEI)/10.13039/501100011033/ and project P07-CVI-02813by Plan Andaluz de Investigación, Desarrollo e Innovación (Junta de Andalucía). The Spanish grantsincluded support from the European Regional Development Fund (ERDF), A way of making Europe.J.A. and M.C.L. are members of the Spanish Carotenoid Network (CaRed), grant RED2022-134577-T,funded by MCIN/AEI/10.13039/501100011033/.
DS Repositorio Institucional de la Universidad de Huelva
RD 15 jul 2026