RT Journal Article
T1 Heteroatom-tagged proteomics of lung cancer and chronic obstructive pulmonary disease human serum reveal alterations in selenoproteins
A1 Callejón Leblic, María Belén
A1 Sánchez Espirilla, Saida
A1 Gómez Ariza, José Luis
A1 García Barrera, Tamara
AB Heteroatom-tagged proteomics allows the absolute quantification of selenoproteins using the heteroatom as a “tag” into a selective and sensitive atomic detector instead of a molecular one. Using this analytical method, about 90% of total selenium in human serum/plasma can be measured as selenoproteins and total selenometabolites and thus, the status of selenium can be determined. Herein, we determined the absolute concentration of selenoproteins in human serum patients with lung cancer (LC) and chronic obstructive pulmonary disease (COPD), a competing cause of morbidity and mortality in smokers as well as an independent risk factor for LC. We conducted an observational study of 154 human serum samples obtained from LC and COPD patients with varying severity of disease, including COPD patients who developed LC during follow-up and healthy controls (HC). Using heteroatom-tagged proteomics, we determined extracellular glutathione peroxidase (eGPx), selenoprotein P (SELENOP), and selenoalbumin (SeAlb). Associations between selenoproteins were also studied as potential biomarkers of disease. The concentration of eGPx was significantly higher in the all-inclusive COPD cohort compared to HC, COPD patients with LC, or those with mild obstructive lung disease, while SELENOP concentration was significantly decreased in LC patients compared to HC and COPD. We found an inverse correlation between SELENOP and SeAlb in HC, but also in LC patients, and especially in patients with COPD and LC. Moreover, we found that eGPx and selenometabolite concentrations were positively associated with LC human serum. Selenoprotein concentrations were altered in COPD and LC when compared to healthy controls suggesting a potential role of the selenoproteome in the diagnosis and/or treatment of these tobacco-related diseases.
PB Elsevier
SN 0026-265X
SN 1095-9149 (electrónico)
YR 2024
FD 2024-01
LK https://hdl.handle.net/10272/23627
UL https://hdl.handle.net/10272/23627
LA eng
NO Callejón-Leblic, B., Sánchez Espirilla, S., Gotera-Rivera, C., Santana, R., Díaz-Olivares, I., María Marín Trigo, J., Casanova Macario, C., Cosio, B. G., Fuster, A., Solanes García, I., de-Torres, J. P., Feu Collado, N., Cabrera Lopez, C., Amado Diago, C., Romero Plaza, A., Padrón Fraysse, L. A., Márquez Martín, E., Marín Royo, M., Balcells Vilarnau, E., … García-Barrera, T. (2024). Heteroatom-tagged proteomics of lung cancer and chronic obstructive pulmonary disease human serum reveal alterations in selenoproteins. In Microchemical Journal (Vol. 199, p. 110033). Elsevier BV. https://doi.org/10.1016/j.microc.2024.110033
NO This work has been supported by the project “Heteroatom-taggedproteomics and metabolomics to study lung cancer. Influence of gutmicrobiota” (Ref.: PY20_00366). Project of Excellence. Regional Ministryof Economy, Knowledge, Business and University, Andalusia, Spain.The authors also thank the grants Ref. 651/2018 and 115/2020 from theSpanish Society of Pneumology and Surgery (SEPAR) and 08/2018 fromthe Association of Pneumology and Thoracic Surgery (Neumosur) thatsupported samples recruitment at the hospitals and biobank registration.The authors also thank Instituto de Salud Carlos III (AES16/01783) andunrestricted funding from Menarini Group and AstraZeneca“. Fundingfor open access charge: Universidad de Huelva / CBUA.
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026