RT Journal Article
T1 Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
A1 Vélez Pérez, Rafael de Fátima
A1 Arias Borrego, Ana
A1 Velasco, Inés
A1 Soldevila, Berta
A1 García Barrera, Tamara
AB Human milk is a crucial source of thyroid hormones, essential for neonatal development. We developed and validated a three-phase hollow-fiber liquid-phase microextraction method, coupled to ultra-high performance liquid chromatography and ion mobility mass spectrometry, to simultaneously quantify eight thyroid hormones, including the chiral forms D-thyroxine and L-thyroxine in human milk. The method showed excellent linearity (R2 > 0.999), low limits of detection (0.7–19.8 μg L-1), and high recoveries (83–114 %). The analysis of 30 human milk samples revealed that D-thyroxine (115.1–157.5 μg L-1) was more abundant than L-thyroxine (47.9–193.6 μg L-1), and reversed triiodothyronine prevailed over triiodothyronine. This is the first report of chiral forms of thyroxine, diiodotirosine, and thyronine in human milk, with potential implications for lipid metabolism and infant thyroid regulation. These findings highlight the method sensitivity and biological relevance, offering a robust tool for future nutritional and endocrine studies.
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/10272/27350
UL https://hdl.handle.net/10272/27350
LA eng
NO Vélez-Pérez, R. de F., Arias-Borrego, A., Velasco, I., Soldevila, B., & García-Barrera, T. (2025). Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine. Food Chemistry, 492. https://doi.org/10.1016/J.FOODCHEM.2025.145542
NO This work was supported by the projects PID2021-123073NB-C21 (Spanish Ministry of Science and Innovation (MCIN). Generaci´on del Conocimiento. MCIN/AEI /10.13039/501100011033/FEDER “Una manera de hacer Europa”), UHU-1256905 and UHU-202009 from the FEDER Andalusian operative program (Ministry of Economic Transformation, Industry, Knowledge and Universities, Andalusia, Spain). This study has been also funded by Instituto de Salud Carlos III (ISCIII) through the project PI22/01350, and co-funded by the European Union. The authors are grateful to FEDER (European Community) for financial support through grants UNHU13-1E-1611 and UNHU15-CE-3140. RFVP gratefully acknowledges the support of the Ministry of Science and Innovation (MCIN) for a FPI predoctoral fellowship (PRE2021-102165). Funding for open access charge: Universidad de Huelva / CBUA.
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jun 2026