RT Journal Article
T1 Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
A1 Villegas Álvarez, Maria Carmen
A1 Callejón Leblic, María Belén
A1 Rodríguez Moro, Gema
A1 Gómez Ariza, José Luis
A1 Arias Borrego, Ana
A1 García Barrera, Tamara
AB A reliable and sensitive analytical approach has been optimized for the extraction of seven polychlorinated biphenyls (PCBs) from human breast milk. Hollow fiber liquid phase microextraction (HF-LPME) was applied for the first time for the extraction and pre-concentration of the analytes. Analytes were separated by gas chromatography with electron capture detector (GC-μECD) for the sensitive detection and mass spectrometry for the unequivocal identification. A rotable central composite design (RCCD) was performed for the multivariate optimization of the method. The best results were obtained at 40 °C during 30 min and 600 rpm of stirring speed using a hollow fiber length of 5 cm and toluene as an extractant phase and salt addition was not required. The detection limits were in the range 7–14 ng L−1 for PCBs. The coefficients of determination of the calibration curves indicated good linearity (R2> 0.96) and the enrichment factors ranged from 74 to 143. This type of study is of great importance due to the deleterious effect that the presence of contaminants can produce in infants health related to the immature character of the defense system. Moreover, exclusive breastfeeding is recommended by neonatologists up to six months of life and as complementary food during the first two years.
PB Elsevier B.V.
SN 0021-9673
SN 1873-3778 (electrónico)
YR 2020
FD 2020
LK https://hdl.handle.net/10272/23241
UL https://hdl.handle.net/10272/23241
LA eng
NO Villegas-Álvarez, M. C., Callejón-Leblic, B., Rodríguez-Moro, G., Gómez-Ariza, J. L., Arias-Borrego, A., & García-Barrera, T. (2020). Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk. In Journal of Chromatography A (Vol. 1626, p. 461381). Elsevier BV. https://doi.org/10.1016/j.chroma.2020.461381
NO The authors thank projects UHU-1256905 (Operative program FEDER-Andalusia 2014–2020, Consejería de Economía, Conocimiento, Empresa y Universidad, Spain) and PGC2018–096608-B-C21 (Ministry of Science and Innovation, Spain). Authors also thank European Community (FEDER) for financial support (UNHU15-CE-3140 and UNHU13–1E-1611). The authors thank Dr. Eduardo García-Soblechero, Celia Pérez-Serralbo and Dr. Elisabeth Gómez-Santos, from the Pediatric Unit of Juan Ramón Jiménez Hospital for the collection of samples.
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jun 2026