RT Journal Article
T1 On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
A1 Blázquez-Carmona, Pablo
A1 Mora Macías, Juan
A1 Pajares Vicente, Antonia
A1 Mármol, Álvaro
A1 Reina Romo, Esther
AB Introduction: Woven bone, a heterogeneous and temporary tissue in bone regeneration, is remodeled by osteoblastic and osteoclastic activity and shaped by mechanical stress to restore healthy tissue properties. Characterizing this tissue at different length scales is crucial for developing micromechanical models that optimize mechanical parameters, thereby controlling regeneration and preventing non-unions.Methods: This study examines the temporal evolution of the mechanical properties of bone distraction callus using nanoindentation, ash analysis, micro-CT for trabecular microarchitecture, and Raman spectroscopy for mineral quality. It also establishes single- and two-parameter power laws based on experimental data to predict tissue-level and bulk mechanical properties.Results: At the macro-scale, the tissue exhibited a considerable increase in bone fraction, controlled by the widening of trabeculae. The Raman mineral-to-matrix ratios increased to cortical levels during regeneration, but the local elastic modulus remained lower. During healing, the tissue underwent changes in ash fraction and in the percentages of Calcium and Phosphorus. Six statistically significant power laws were identified based on the ash fraction, bone fraction, and chemical and Raman parameters.Discussion: The microarchitecture of woven bone plays a more significant role than its chemical composition in determining the apparent elastic modulus of the tissue. Raman parameters were demonstrated to provide more significant power laws correlations with the micro-scale elastic modulus than mineral content from ash analysis.
PB Frontiers Media
SN 2296-4185 (electrónico)
YR 2024
FD 2024-10
LK https://hdl.handle.net/10272/24738
UL https://hdl.handle.net/10272/24738
LA eng
NO Blázquez-Carmona, P., Mora-Macías, J., Pajares, A., Mármol, Á., & Reina-Romo, E. (2024). On the influence of structural and chemical properties on the elastic modulus of woven bone under healing. In Frontiers in Bioengineering and Biotechnology (Vol. 12). Frontiers Media SA. https://doi.org/10.3389/fbioe.2024.1476473
NO The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. Grant PID 2020-113790RB-I00 funded by MICIU/AEI/10.13039/501100011033.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026