RT Journal Article
T1 Synergistic antifungal activity against Candida albicans between voriconazole and cyclosporine a loaded in polymeric nanoparticles
A1 Martín Herrera, Victoria Isabel
A1 López López, Manuel
A1 Moyá, María Luisa
AB The goal of this work is to investigate if the synergistic antifungal activity between cyclosporine A, CsA, andvoriconazole, VRZ, increases when both drugs are encapsulated in a nanocarrier as compared when they are free.The preparation and characterization of blank and VRZ and CsA loaded polymeric based PLGA nanoparticles(PLGA, PLGA-PEG, and PLGA+PEG) was a necessary previous step. Using the more suitable NPs, those of PLGA,the antifungal susceptibility tests performed with VRZ-loaded PLGA NPs, show no significant increase of theantifungal activity in comparison to that of free VRZ. However, the synergistic behavior found for the(VRZ+CsA)-loaded PLGA NPs was fourfold stronger than that observed for the two free drugs together. On theother hand, the investigation into the suppression of C. albicans biofilm formation showed that blank PLGA NPsinhibit the biofilm formation at high NPs concentrations. However, a minor effect or even a slight biofilm increaseformation was observed at low and moderate NPs concentrations. Therefore, the enhancement of thebiofilm inhibition found for the three tested treatments (CsA alone, VRZ alone, and VRZ+CsA) when comparingfree and encapsulated drugs, within the therapeutic window, can be attributed to the drug encapsulationapproach. Indeed, polymeric PLGA NPs loaded with CsA, VRZ, or VRZ+CsA are more effective at inhibiting theC. albicans biofilm growth than their free counterparts.
PB Elsevier
SN 0378-5173
SN 1873-3476 (electrónico)
YR 2024
FD 2024-08
LK https://hdl.handle.net/10272/24726
UL https://hdl.handle.net/10272/24726
LA eng
NO Martín, V., de la Haba, R. R., López-Cornejo, P., López-López, M., Antonio Lebrón, J., Bernal, E., Baeza, N., Ruiz, S., José Ostos, F., Merino-Bohorquez, V., Chevalier, S., Lesouhaitier, O., Tahrioui, A., José Montes, F., Sánchez-Carrasco, T., & Luisa Moyá, M. (2024). Synergistic antifungal activity against Candida albicans between voriconazole and cyclosporine a loaded in polymeric nanoparticles. In International Journal of Pharmaceutics (Vol. 664, p. 124593). Elsevier BV. https://doi.org/10.1016/j.ijpharm.2024.124593
NO This work was financied by the Consejería de Economía, Conocimiento,Empresas y Universidades de la Junta de Andalucía (FQM-206, FQM-274), the European Union (Feder Funds), and by the University of Seville (Ayudas Servicios Generales de la Universidad, CITIUS, 2024/00000387, 2024/00000395). The authors acknowledge the postdoctoral grant of F.J. Ostos (PAIDI-DOCTOR, Junta de Andalucía with European Social Fund, DOC_00963).
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026