RT Journal Article
T1 Absolute quantification of selenoproteins and selenometabolites in lung cancer human serum by column switching coupled to triple quadrupole inductively coupled plasma mass spectrometry
A1 Callejón Leblic, María Belén
A1 Rodríguez Moro, Gema
A1 Arias Borrego, Ana
A1 Pereira Vega, Antonio
A1 Gómez Ariza, José Luis
A1 García Barrera, Tamara
K1 Química analítica
AB Una de las causas más importantes de la alta tasa de mortalidad y la baja esperanza de vida del cáncer de pulmón es la detección en estadios avanzados. Por ello, existe una necesidad urgente de un diagnóstico precoz y de la búsqueda de nuevos biomarcadores selectivos. El selenio es un componente importante de las selenoproteínas y un potente antioxidante capaz de proteger frente al cáncer. En este trabajo se ha realizado por primera vez la cuantificación absoluta de selenio en selenoproteínas y el contenido total en selenometabolitos en suero de pacientes con cáncer de pulmón (CL) y controles sanos (HC). Para ello, se desarrolló un método de especiación simultánea de selenoproteínas mediante cromatografía de exclusión por tamaño (SEC) y cromatografía de afinidad (AF) con detección por ICP-QQQ-MS, y cuantificación por dilución isotópica (IDA) (SEC-AF-HPLC-SUID-ICP-QQQ-MS) para determinar la concentración de selenio en eGPx, SEPP1 y SeAlb, así como selenometabolitos totales, para encontrar alteraciones que puedan servir como biomarcadores de esta enfermedad. De la misma forma, se desarrolló un método basado en cromatografía de intercambio aniónico acoplado a ICP-QQQ-MS para cuantificar selenometabolitos (SeCys2, SeMeSeCys, SeMet, selenito y selenato) en las mismas muestras de suero.
AB One of the most important causes of the high mortality rate and low life expectancy of lung cancer is the detection at advanced stages. Thus, there is an urgent need for early diagnosis and the search of new selective biomarkers. Selenium is an important constituent of selenoproteins and a powerful antioxidant able to protect against cancer. In this work, the absolute quantification of selenium in selenoproteins and the total content in selenometabolites has been performed for the first time in serum from lung cancer patients (LC) and healthy controls (HC). To this end, a method for the simultaneous speciation of selenoproteins using size exclusion chromatography (SEC) and affinity chromatography (AF) with detection by ICP-QQQ-MS, and quantification by isotopic dilution (IDA) (SEC-AF-HPLC-SUID-ICP-QQQ-MS) was developed to determine the selenium concentration in eGPx, SEPP1 and SeAlb, as well as total selenometabolites, to find alterations that may serve as biomarkers of this disease. In the same way, a method based on anion-exchange chromatography coupled to ICP-QQQ-MS was developed to quantify selenometabolites (SeCys2, SeMeSeCys, SeMet, selenite and selenate) in the same LC and HC serum samples. The results showed that the averaged concentrations of selenium in eGPx, SeAlb and selenite were significantly higher in LC patients (LC (eGPx: 21.24 ± 0.77 ng g−1; SeAlb: 49.56 ± 3.16 ng g−1 and Se(IV): 6.20 ± 1.22 ng g−1) than in HC group (eGPx: 16.96 ± 0.53 ng g−1; SeAlb: 38.33 ± 2.66 ng g−1 and Se(IV): 3.56 ± 0.55 ng g−1). In addition, the ratios between selenoproteins and selenometabolites have been calculated for the first to study their potential use as LC biomarkers. The rates eGPx/SEPP1, SEPP1/SeAlb, eGPx/Se(IV) and SEPP1/Se(IV) were significantly different between LC and HC groups.
PB Elsevier
SN 1873-3778
YR 2020
FD 2020-05-24
LK https://hdl.handle.net/10272/24895
UL https://hdl.handle.net/10272/24895
LA eng
NO Belén Callejón-Leblic, Gema Rodríguez-Moro, Ana Arias-Borrego, Antonio Pereira-Vega, José Luis Gómez-Ariza, Tamara García-Barrera, Absolute quantification of selenoproteins and selenometabolites in lung cancer human serum by column switching coupled to triple quadrupole inductively coupled plasma mass spectrometry, Journal of Chromatography A, Volume 1619, 2020, 460919, ISSN 0021-9673, https://doi.org/10.1016/j.chroma.2020.460919.
NO This research was funded by the projects PGC2018-096608-B-C21 from the Spanish Ministry of Economy and Competitiveness, and P12-FQM-0442 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). Projects Neumosur (8/2012 and 9/2015) and SEPAR (124/2012 and 091/2016). Finally, authors are grateful to FEDER (European Community) for financial support, grants number UNHU13-1E-1611 and UNHU15-CE-3140.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026