RT Journal Article
T1 Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
A1 León Vaz, Antonio
A1 Romero, Luis C.
A1 Gotor, Cecilia
A1 León Bañares, Rosa María
A1 Vigara Fernández, Javier
AB Cadmium is one of the most common heavy metals in contaminated aquatic environments and one of the mosttoxic contaminants for phytoplankton. Nevertheless, there are not enough studies focused on the effect of thismetal in algae. Through a proteomic approach, this work shows how Cd can alter the growth, cell morphologyand metabolism of the microalga Chlorella sorokiniana. Using the sequential window acquisition of all theoreticalfragment ion spectra mass spectrometry (SWATH-MS), we concluded that exposure of Chlorella sorokiniana to250 μM Cd2+ for 40 h caused downregulation of different metabolic pathways, such as photosynthesis, oxidativephosphorylation, glycolysis, TCA cycle and ribosomal proteins biosynthesis. However, photorespiration, antioxidantenzymes, gluconeogenesis, starch catabolism, and biosynthesis of glutamate, cysteine, glycine and serinewere upregulated, under the same conditions. Finally, exposure to Cd also led to changes in the metabolism ofcarotenoids and lipids. In addition, the high tolerance of Chlorella sorokiniana to Cd points to this microalga as apotential microorganism to be used in bioremediation processes.
PB Elsevier
SN 0147-6513
YR 2020
FD 2020-09
LK http://hdl.handle.net/10272/19142
UL http://hdl.handle.net/10272/19142
LA eng
NO León Vaz, A., Romero, L. C., Gotor, C., León  Bañares, R., & Vigara Fernández, J. (2020). Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study. Ecotoxicology and Environmental Safety, 207, 111301. DOI: https://doi.org/10.1016/j.ecoenv.2020.111301
NO This work was supported by European governments (INTERREG VA- POCTEP- 2014–2020; 0055_ALGARED_PLUS_5_E) and  the  European Regional Development Fund through the  Agencia Estatal de  Inves-tigaci ́on grants (PID 2019-109785 GB-I00 and PID 2019-110438RB- C22). We want to thank Dr. Rocío Rodríguez for the proteomics service.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026