RT Journal Article
T1 Application of a novel metabolomic approach based on atmospheric pressure photoionization mass spectrometry using flow injection analysis for the study of Alzheimer׳s disease
A1 González Domínguez, Raúl
A1 García Barrera, Tamara
A1 Gómez Ariza, José Luis
AB The use of atmospheric pressure photoionization is not widespread in metabolomics, despite its considerable potential for the simultaneous analysis of compounds with diverse polarities. This work considers the development of a novel analytical approach based on flow injection analysis and atmospheric pressure photoionization mass spectrometry for rapid metabolic screening of serum samples. Several experimental parameters were optimized, such as type of dopant, flow injection solvent, and their flows, given that a careful selection of these variables is mandatory for a comprehensive analysis of metabolites. Toluene and methanol were the most suitable dopant and flow injection solvent, respectively. Moreover, analysis in negative mode required higher solvent and dopant flows (100 µl min−1 and 40 µl min−1, respectively) compared to positive mode (50 µl min−1 and 20 µl min−1). Then, the optimized approach was used to elucidate metabolic alterations associated with Alzheimer׳s disease. Thereby, results confirm the increase of diacylglycerols, ceramides, ceramide-1-phosphate and free fatty acids, indicating membrane destabilization processes, and reduction of fatty acid amides and several neurotransmitters related to impairments in neuronal transmission, among others. Therefore, it could be concluded that this metabolomic tool presents a great potential for analysis of biological samples, considering its high-throughput screening capability, fast analysis and comprehensive metabolite coverage.
PB Elsevier
SN 0039-9140
SN 1873-3573 (electrónico)
YR 2015
FD 2015
LK http://hdl.handle.net/10272/14678
UL http://hdl.handle.net/10272/14678
LA eng
NO González Domínguez, R., García Barrera, T., Gómez Ariza, J.L.: "Application of a novel metabolomic approach based on atmospheric pressure photoionization mass spectrometry using flow injection analysis for the study of Alzheimer׳s disease". Talanta. Vol. 131, p´gs. 480-489, (2015). DOI: 10.1016/j.talanta.2014.07.075
NO This work was supported by the projects CTM2012-38720-C03-01 from the Ministerio de Ciencia e Innovacion and P012-FQM-0442 and P009-FQM-4659 from the Consejeria de Innovacion, Ciencia y Empresa (Junta de Andalucia). Raul Gonzalez Dominguez thanks the Ministerio de Educacion (Grant no. AP2010-4278) for a predoctoral scholarship.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026