RT Journal Article
T1 Combination of metabolomic and phospholipid-profiling approaches for the study of Alzheimer's disease
A1 González Domínguez, Raúl
A1 García Barrera, Tamara
A1 Gómez Ariza, José Luis
AB Alzheimer's disease is closely related to abnormal metabolism of phospholipids from neural membranes, so that the study of their dyshomeostasis could be of great interest for the discovery of potential biomarkers for diagnosis and disease monitoring. In this work, it has been developed a metabolomic multi-platform for the characterization of phospholipid alterations occurring in serum from Alzheimer's disease patients. For this purpose, we performed a metabolomic screening by direct infusion mass spectrometry and profiling analysis by reversed phase ultra-high performance liquid chromatography with complementary detection by molecular and atomic mass spectrometry, which allowed combining the high-throughput capability of shotgun metabolomics and the targeted character of profiling approaches. Thus significant changes were detected in the levels of several molecular species of phosphatidylcholines, phosphatidylethanolamines, plasmenylcholines, plasmenylethanolamines and different classes of lysophospholipids, which provided a global vision of the possible factors triggering membrane breakdown. In this sense, alterations of phospholipids metabolism appears to have a multifactorial origin involving overactivation of phospholipases, increased anabolism of lysophospholipids, peroxisomal dysfunction, imbalances in the levels of saturated/unsaturated fatty acids contained in the structure of phospholipids and oxidative stress.
PB Elsevier
SN 1874-3919
SN 1876-7737 (electrónico)
YR 2014
FD 2014
LK http://hdl.handle.net/10272/14482
UL http://hdl.handle.net/10272/14482
LA eng
NO González Domínguez, R., García Barrera, T., Gómez Ariza, J.L.: "Combination of metabolomic and phospholipid-profiling approaches for the study of Alzheimer's disease". Journal of Proteomics. Vol. 104, págs. 37-47, (2014). DOI: 10.1016/j.jprot.2014.01.014
NO This work was supported by the projects CTM2012-38720-C03-01 from the Ministerio de Ciencia e Innovacion and P008-FQM-3554 and P009-FQM-4659 from the Consejeria de Irmovacion, Ciencia y Empresa (Junta de Andalucia). REG]. Gonzalez Dominguez thanks the Ministerio de Educacion for a predoctoral scholarship. The authors also thank to Dr. Alberto Blanco and Carlos Salgado from Hospital Juan Ramon Jimenez for providing serum samples.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026