RT Journal Article
T1 Biochemical Characterization of the Amylase Activity from the New Haloarchaeal Strain Haloarcula sp. HS Isolated in the Odiel Marshlands
A1 Gómez Villegas, Patricia
A1 Vigara Fernández, Javier
A1 Romero, Luis
A1 Gotor, Cecilia
A1 Raposo, Sara
A1 Gonçalves, Brígida
A1 León Bañares, Rosa María
AB Alpha-amylases are a large family of  ,1-4-endo-glycosyl hydrolases distributed in allkingdoms of life. The need for poly-extremotolerant amylases encouraged their search in extremeenvironments, where archaea become ideal candidates to provide new enzymes that are able to workin the harsh conditions demanded in many industrial applications. In this study, a collection ofhaloarchaea isolated from Odiel saltern ponds in the southwest of Spain was screened for their amylaseactivity. The strain that exhibited the highest activity was selected and identified as Haloarcula sp.HS.We demonstrated the existence in both, cellular and extracellular extracts of the new strain, offunctional  -amylase activities, which showed to be moderately thermotolerant (optimum around60 ◦C), extremely halotolerant (optimum over 25% NaCl), and calcium-dependent. The tryptic digestionfollowed by HPLC-MS/MS analysis of the partially purified cellular and extracellular extractsallowed to identify the sequence of three alpha-amylases, which despite sharing a low sequence identity,exhibited high three-dimensional structure homology, conserving the typical domains and mostof the key consensus residues of  -amylases. Moreover, we proved the potential of the extracellular -amylase from Haloarcula sp. HS to treat bakery wastes under high salinity conditions.
PB MDPI
SN 2079-7737
YR 2021
FD 2021-04
LK http://hdl.handle.net/10272/19824
UL http://hdl.handle.net/10272/19824
LA eng
NO Gómez Villegas, P., Vigara Fernández, J., Romero, L., Gotor, C., Raposo, S., Gonçalves, B. & León Bañares, R. M. (2021). Biochemical Characterization of the Amylase Activity from the New Haloarchaeal Strain Haloarcula sp. HS Isolated in the Odiel Marshlands. Biology, 10, 337. DOI: https://doi.org/10.3390/ biology10040337
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026