RT Journal Article
T1 Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery
A1 Lebrón, José Antonio
A1 López López, Manuel
A1 García Calderón, Clara B.
A1 Rosado, Ivan V.
A1 Balestra, Fernando R.
A1 Huertas, Pablo
A1 Rodik, Roman V.
A1 Kalchenko, Vitaly I.
A1 Bernal, Eva
A1 Moyá, María Luisa
A1 López Cornejo, Pilar
A1 Ostos, Francisco José
AB The formation of calixarene-based liposomes was investigated, and the characterization ofthese nanostructures was carried out using several techniques. Four amphiphilic calixarenes wereused. The length of the hydrophobic chains attached to the lower rim as well as the nature of thepolar group present in the upper rim of the calixarenes were varied. The lipid bilayer was formedwith one calixarene and with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine,DOPE. The cytotoxicity of the liposomes for various cell lines was also studied. From the resultsobtained, the liposomes formed with the least cytotoxic calixarene, (TEAC12)4, were used as nanocarriers of both nucleic acids and the antineoplastic drug doxorubicin, DOX. Results showed that(TEAC12)4/DOPE/p-EGFP-C1 lipoplexes, of a given composition, can transfect the genetic material,although the transfection efficiency substantially increases in the presence of an additional amount ofDOPE as coadjuvant. On the other hand, the (TEAC12)4/DOPE liposomes present a high doxorubicinencapsulation efficiency, and a slow controlled release, which could diminish the side effects ofthe drug
PB MDPI
SN 1999-4923 (electrónico)
YR 2021
FD 2021
LK http://hdl.handle.net/10272/20275
UL http://hdl.handle.net/10272/20275
LA eng
NO Lebrón, J. A., López-López, M., García-Calderón, C. B., V. Rosado, I., Balestra, F. R., Huertas, P., Rodik, R. V., Kalchenko, V. I., Bernal, E., Moyá, M. L., López-Cornejo, P., & Ostos, F. J. (2021). Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery. In Pharmaceutics (Vol. 13, Issue 8, p. 1250). MDPI AG. https://doi.org/10.3390/pharmaceutics13081250
NO This work was financed by the Consejería de Conocimiento, Innovación y Universidadesde la Junta de Andalucía (FQM-206, FQM-274, and PY20-01234), the VI Plan Propio Universidad deSevilla (PP2019/00000748), RTI2018-100692-B-100; P18-RT-1271; PI18-0005-2018; VI-PP AY.SUPLEM2019; RYC-2015-18670, The R+D+I grant PID2019-104195G from the Spanish Ministry of Science andInnovation-Agencia Estatal de Investigación/10.13039/501100011033 (P.H.) and the European Union(Feder Funds). The authors thank the University of Seville for the grant VPPI-US. J.A.L. also thanksthe Fundación ONCE funded by the Fondo Social Europeo
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026