RT Journal Article
T1 Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
A1 Gojkovic, Živan
A1 Vílchez Lobato, Carlos
A1 Torronteras Santiago, Rafael
A1 Vigara Fernández, Javier
A1 Gómez Jacinto, Verónica
A1 Janzer, Nora
A1 Gómez Ariza, José Luis
A1 Márová, Ivana
A1 Garbayo Nores, Inés
AB The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of thegreen alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg⋅L−1 (212 μ M)decreased growth rates for about 25% compared to control. A selenate EC50 value of 45mg⋅L−1 (238.2 μ M) was determined. Resultsshowed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half.Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity.Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of aprotein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of thecorresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able toaccumulate up to 140mg⋅kg−1 of SeMet in 120h of cultivation.This paper shows that Chlorella sorokiniana biomass can be enrichedin the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activityintact, if exposed to suitable sublethal concentrations of Se.
PB Hindawi Publishing Corporation
SN 1537-744X
YR 2014
FD 2014
LK http://hdl.handle.net/10272/8383
UL http://hdl.handle.net/10272/8383
LA eng
NO Gojkovic, Ž., Vílchez Lobato, C., Torronteras Santiago, R., Vigara Fernández, J., Gómez Jacinto, V., Janzer, N., Gómez Ariza, J.L., Márová, I., Garbayo Nores, I.: "Effect of Selenate on Viability and SelenomethionineAccumulation of Chlorella sorokiniana Grown in Batch Culture". ScientificWorld Journal. Volume 2014 (2014), Article ID 401265. ISSN 1537-744X
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026