RT Journal Article
T1 Ultrasound-Based Recovery of Anti-Inflammatory and Antimicrobial Extracts of the Acidophilic Microalga Coccomyxa onubensis
A1 Ruiz Domínguez, María del Carmen
A1 Robles Garrido, María
A1 Martín Gordillo, Lidia
A1 Beltrán Martín, Álvaro
A1 Gava, Riccardo
A1 Cuaresma Franco, María
A1 Navarro Roldán, Francisco Juan
A1 Vílchez Lobato, Carlos
A1 Ruiz Domínguez, María del Carmen
AB In the present study, the recovery of valuable molecules of proven anti-inflammatory and antimicrobial activity of the acidophilic microalga Coccomyxa onubensis (C. onubensis) were evaluated using green technologies based on ultrasound-assisted extraction (UAE). Using a factorial design (3 × 2) based on response surface methodology and Pareto charts, two types of ultrasonic equipment (bath and probe) were evaluated to recover valuable compounds, including the major terpenoid of C. onubensis, lutein, and the antimicrobial activity of the microalgal extracts obtained under optimal ultrasound conditions (desirability function) was evaluated versus conventional extraction. Significant differences in lutein recovery were observed between ultrasonic bath and ultrasonic probe and conventional extraction. Furthermore, the antimicrobial activity displayed by C. onubensis UAE-based extracts was greater than that obtained in solvent-based extracts, highlighting the effects of the extracts against pathogens such as Enterococcus hirae and Bacillus subtilis, followed by Staphylococcus aureus and Escherichia coli. In addition, gas chromatography–mass spectrometry was performed to detect valuable anti-inflammatory and antimicrobial biomolecules present in the optimal C. onubensis extracts, which revealed that phytol, sterol-like, terpenoid, and even fatty acid structures could also be responsible for the antibacterial activities of the extracts. Moreover, UAE displayed a positive effect on the recovery of valuable molecules, improving biocidal effects. Our study results facilitate the use of green technology as a good tool in algal bioprocess engineering, improving energy consumption and minimizing environmental impacts and process costs, as well as provide a valuable product for applications in the field of biotechnology.
PB MDPI
SN 1660-3397 (electrónico)
YR 2023
FD 2023-08
LK https://hdl.handle.net/10272/22448
UL https://hdl.handle.net/10272/22448
LA eng
NO Ruiz-Domínguez, M. C., Robles, M., Martín, L., Beltrán, Á., Gava, R., Cuaresma, M., Navarro, F., & Vílchez, C. (2023). Ultrasound-Based Recovery of Anti-Inflammatory and Antimicrobial Extracts of the Acidophilic Microalga Coccomyxa onubensis. In Marine Drugs (Vol. 21, Issue 9, p. 471). MDPI AG. https://doi.org/10.3390/md21090471
NO This work was partially supported by the Andalusian Research, Development and Innovation Plan (Junta de Andalucía, Spain) with FEDER funds (Project P20_00930) and by the University of Huelva through a grant obtained in the program “Requalitication of the Spanish Universitary System 2021-2023, María Zambrano” (Real Decreto 289/2021, 20 April and Order UNI/551/2021, 26 May) and “IV Convocatoria de Micro-proyectos-Cátedra de la Provincia UHU 2023”.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026