RT Generic
T1 Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine [Dataset]
A1 Vélez Pérez, Rafael de Fátima
A1 Arias Borrego, Ana
A1 Velásco, Inés
A1 Soldevila, Berta
A1 García Barrera, Tamara
AB Human milk is a crucial source of thyroid hormones, essential for neonatal development. We developed and validated a three-phase hollow-fiber liquid-phase microextraction method, coupled to ultra-high performance liquid chromatography and ion mobility mass spectrometry, to simultaneously quantify eight thyroid hormones, including the chiral forms D-thyroxine and L-thyroxine in human milk. The method showed excellent linearity (R2 > 0.999), low limits of detection (0.7–19.8 μg L−1), and high recoveries (83–114 %). The analysis of 30 human milk samples, revealed that D-thyroxine (115.1–157.5 μg L−1) was more abundant than L-thyroxine (47.9–193.6 μg L−1), and reversed triiodothyronine prevailed over triiodothyronine. This is the first report of chiral forms of thyroxine, diiodotirosine, and thyronine in human milk, with potential implications for lipid metabolism and infant thyroid regulation. These findings highlight the method sensitivity and biological rele­ vance, offering a robust tool for future nutritional and endocrine studies.
PB Elsevier
YR 2025
FD 2025-07-11
LK https://hdl.handle.net/10272/27342
UL https://hdl.handle.net/10272/27342
LA eng
NO Vélez-Pérez, R. de F., Arias-Borrego, A., Velasco, I., Soldevila, B., & García-Barrera, T. (2025). Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine. Food Chemistry, 492. https://doi.org/10.1016/J.FOODCHEM.2025.145542.   [Dataset]. AM (Depósito de Investigación de la Universidad de Huelva).
NO Este repositorio incluye dos conjuntos de datos complementarios al artículo (DOI: 10.1016/j.foodchem.2025.145542). El primero corresponde a una tabla con los resultados de las concentraciones de hormonas obtenidas en todas las muestras analizadas, mientras que el segundo contiene tablas y gráficos con los resultados de la optimización del método de extracción empleado en dicho estudio.
DS Repositorio Institucional de la Universidad de Huelva
RD 3 jul 2026