@article{10272/23150,
year = {2015},
month = {2},
url = {https://hdl.handle.net/10272/23150},
abstract = {We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.},
organization = {This work was financed by the Swedish Research Council VR (grant 622-2010-280), the European Research Council (ERC FP7/2007-2013 grant no. 203952), and the BBSRC (sLOLA grant BB/J001694/1 "extending the boundaries of nucleic acid chemistry”). The authors thank Melina Gilbert Gatty for assistance with the SPC measurements and Niklas Bosaeus for running the electrophoresis gels.},
publisher = {American Chemical Society (ACS)},
title = {Reversible Energy-Transfer Switching on a DNA Scaffold},
doi = {10.1021/ja512416n},
author = {Bälter, Magnus and Hammarson, Martin and Remón Ruiz, Patricia and Li, Shiming and Gale, Nittaya and Brown, Tom and Andréasson, Joakim},
}