RT Journal Article
T1 Reversible Energy-Transfer Switching on a DNA Scaffold
A1 Bälter, Magnus
A1 Hammarson, Martin
A1 Remón Ruiz, Patricia
A1 Li, Shiming
A1 Gale, Nittaya
A1 Brown, Tom
A1 Andréasson, Joakim
AB We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.
PB American Chemical Society (ACS)
SN 0002-7863
SN 1520-5126 (electrónico)
YR 2015
FD 2015-02
LK https://hdl.handle.net/10272/23150
UL https://hdl.handle.net/10272/23150
LA eng
NO Bälter, M., Hammarson, M., Remón, P., Li, S., Gale, N., Brown, T., & Andréasson, J. (2015). Reversible Energy-Transfer Switching on a DNA Scaffold. In Journal of the American Chemical Society (Vol. 137, Issue 7, pp. 2444–2447). American Chemical Society (ACS). https://doi.org/10.1021/ja512416n
NO This work was financed by the Swedish Research Council VR (grant 622-2010-280), the European Research Council (ERC FP7/2007-2013 grant no. 203952), and the BBSRC (sLOLA grant BB/J001694/1 "extending the boundaries of nucleic acid chemistry”). The authors thank Melina Gilbert Gatty for assistance with the SPC measurements and Niklas Bosaeus for running the electrophoresis gels.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026